克尔斯滕和哈维鼠肉瘤病毒基因组的大鼠序列：性质、起源及在大鼠肿瘤RNA中的表达
Rat sequences of the Kirsten and Harvey murine sarcoma virus genomes: nature, origin, and expression in rat tumor RNA.
作者信息
Anderson G R, Robbins K C
出版信息
J Virol. 1976 Feb;17(2):335-51. doi: 10.1128/JVI.17.2.335-351.1976.
Two murine sarcoma viruses, the Kirsten and the Harvey, were isolated by passage of mouse type C leukemia viruses through rats. These sarcoma viruses have genomes containing portions of their parental type C mouse leukemia virus genomes, in stable association with specific rat cellular sequences that we find to be quite likely not those of a rat type C leukemia virus. To determine if these murine sarcoma viruses provide a model relevant to the events occurring in spontaneous tumors, we have hybridized DNA and RNA prepared from rat tumors and normal rat tissues to [3H]DNA prepared from the Kirsten murine sarcoma virus. We have also hybridized these rat tissue nucleic acids to [3H]DNA prepared from a respresentative endogenous rat type C leukemia virus, the WFU (Wistar-Furth). Sarcoma-viral rat cellular sequences and endogenous rat leukemia viral sequences were detected in the DNA of both tumor and normal tissues, with no evidence of either gene amplification or additional sequences being present in tumor DNA. Sarcoma-viral rat cellular sequences and endogenous rat leukemia viral sequences were detected at elevated concentrations in the RNA of many rat tumors and in specific groups of normal tissues.
两种鼠肉瘤病毒，即柯斯顿病毒和哈维病毒，是通过将小鼠C型白血病病毒在大鼠体内传代而分离得到的。这些肉瘤病毒的基因组包含其亲本C型小鼠白血病病毒基因组的部分片段，并与特定的大鼠细胞序列稳定结合，我们发现这些序列很可能不是大鼠C型白血病病毒的序列。为了确定这些鼠肉瘤病毒是否提供了一个与自发肿瘤中发生的事件相关的模型，我们将从大鼠肿瘤和正常大鼠组织中制备的DNA和RNA与从柯斯顿鼠肉瘤病毒制备的[3H]DNA进行了杂交。我们还将这些大鼠组织核酸与从一种代表性的内源性大鼠C型白血病病毒WFU（威斯塔-富思）制备的[3H]DNA进行了杂交。在肿瘤和正常组织的DNA中均检测到肉瘤病毒-大鼠细胞序列和内源性大鼠白血病病毒序列，没有证据表明肿瘤DNA中存在基因扩增或其他额外序列。在许多大鼠肿瘤的RNA以及特定组别的正常组织中，检测到肉瘤病毒-大鼠细胞序列和内源性大鼠白血病病毒序列的浓度升高。
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