Reynolds R K, van de Ven W J, Stephenson J R
J Virol. 1978 Nov;28(2):665-70. doi: 10.1128/JVI.28.2.665-670.1978.
The genomic RNA of Abelson leukemia virus (AbLV) has been purified and translated in Xenopus laevis oocytes. The primary AbLV-specific protein synthesized is a polyprotein corresponding in molecular weight and immunological properties to a previously described p15 and p12 containing 110,000- to 130,000-molecular-weight polyprotein expressed in AbLV-transformed cells. In contrast, translation of woolly monkey sarcoma virus genomic RNA resulted in symthesis of a 55,000-molecular-weight polyprotein consisting of woolly helper virus p30, p15, and p12. These findings demonstrate the value of the X. laevis oocyte in vitro system for studies of translational products of replication-defective transforming viruses and establish the virus-coded nature of the nonstructural component of the 110,000- to 130,000-molecular-weight polyprotein expressed in AbLV-transformed cells.
阿贝尔森白血病病毒（AbLV）的基因组RNA已被纯化，并在非洲爪蟾卵母细胞中进行翻译。合成的主要AbLV特异性蛋白是一种多聚蛋白，其分子量和免疫特性与先前描述的存在于AbLV转化细胞中、包含分子量为110,000至130,000的p15和p12的多聚蛋白相对应。相比之下，绒毛猴肉瘤病毒基因组RNA的翻译产生了一种分子量为55,000的多聚蛋白，该多聚蛋白由绒毛辅助病毒的p30、p15和p12组成。这些发现证明了非洲爪蟾卵母细胞体外系统在研究复制缺陷型转化病毒翻译产物方面的价值，并确定了在AbLV转化细胞中表达的分子量为110,000至130,000的多聚蛋白非结构成分的病毒编码性质。
