高效液相色谱法测定职业接触苯工人尿中反式、反式粘康酸的排泄量。

High-performance liquid chromatographic determination of urinary trans, trans-muconic acid excreted by workers occupationally exposed to benzene.

作者信息

Hu Xia-Min, Song Shi-Zhen, Ye Fang-Li, Liu Li-Wen

机构信息

Department of Pharmacology, Medical College of Wuhan University of Science and Technology, Wuhan 430080, Hubei, China.

出版信息

Biomed Environ Sci. 2006 Aug;19(4):292-6.

PMID:17044647

Abstract

OBJECTIVE

To investigate the relationship between trans, trans-muconic acid (ttMA) as benzene metabolite of occupational workers and benzene concentration in air.

METHODS

A rapid and sensitive high-performance liquid chromatography was developed to determine the level of urinary ttMA. ttMA was extrated from urinary samples in liquid-liquid phase a ODS (2) (5u) column (phi 4.6 mm x 150 mm) and detected at wavelength 264 nm in a UV detector using vanillic acid as an internal standard. The mobile phase was acetaticacid/tetrahydrofuran/methanol/water (v/v, 1:2:10:87). The method was validated with 56 urine samples collected from occupationally benzene-exposed individuals.

RESULTS

A correlation coefficient (r = 0.9963) was found for ttMA ranging 0.10-10.00 microg/mL. The limit of detection was 0.10 microg/mL. The recovery and reproducibility were generally over 90%. There was a positive correlation between ttMA and benzene level in air. The equation was Y = 0.859 + 0.108C (before work, r = 0.6200) or Y = 1.980 + 0.179C (after work, r = 0.7930).

CONCLUSION

This method can be used to determine and control the level of urinary ttMA in those who are occupationally exposed to benzene.

摘要

目的

探讨职业接触苯工人的苯代谢产物反，反-粘康酸（ttMA）与空气中苯浓度之间的关系。

方法

建立了一种快速灵敏的高效液相色谱法测定尿中ttMA水平。采用液-液萃取法从尿样中提取ttMA，在ODS(2)(5u)柱（内径4.6mm×150mm）上分离，以香草酸为内标，在紫外检测器264nm波长处检测。流动相为乙酸/四氢呋喃/甲醇/水（体积比1:2:10:87）。用从职业接触苯的个体收集的56份尿样对该方法进行验证。

结果

ttMA在0.10 - 10.00μg/mL范围内的相关系数（r = 0.9963）。检测限为0.10μg/mL。回收率和重现性一般均超过90%。尿中ttMA与空气中苯水平呈正相关。回归方程为Y = 0.859 + 0.108C（班前，r = 0.6200）或Y = 1.980 + 0.179C（班后，r = 0.7930）。