Kiyici Sinem, Erturk Erdinc, Budak Ferah, Ersoy Canan, Tuncel Ercan, Duran Cevdet, Oral Barbaros, Sigirci Deniz, Imamoglu Sazi
Department of Endocrinology and Metabolism, Medical Faculty, Uludag University, Bursa, Turkey.
Arch Med Res. 2006 Nov;37(8):998-1003. doi: 10.1016/j.arcmed.2006.06.002.
Monocyte chemoattractant protein (MCP)-1 is suggested to be implicated in the pathogenesis of diabetic nephropathy by activating and recruiting monocytes to the glomerulus via regulation of adhesion molecule expressions. The aim of this study was to test potential associations between serum concentrations of MCP-1, monocyte expression of Mac-1 and LFA-1 and nephropathy in type 1 diabetes mellitus.
Serum MCP-1 levels and expression of monocyte adhesion molecules in 51 type 1 diabetic patients with and without diabetic nephropathy were compared with matched 15 healthy control subjects. Concentrations of serum MCP-1 were determined by enzyme-linked immunosorbent assays whereas monocyte expression of adhesion molecules Mac-1 and LFA-1 was measured by flow cytometry.
Serum MCP-1 levels and expression of Mac-1, but not LFA-1, were significantly higher in diabetic patients compared with controls. The mean serum MCP-1 level was 137.2 +/- 71.4 pg/mL in control patients, whereas it was 246.2 +/- 114.9 pg/ml in diabetic patients (p = 0.002). Serum MCP-1 levels were positively correlated with HbA1c and plasma fasting glucose levels. There was no difference in serum levels of MCP-1 and expression of monocyte adhesion molecules between type 1 diabetic patients with and without diabetic nephropathy.
In type 1 diabetic patients, the levels of circulating MCP-1 concentration and expression of Mac-1 is mostly influenced by glycemic control rather than the existence of diabetic nephropathy.
单核细胞趋化蛋白（MCP）-1被认为通过调节黏附分子表达激活并将单核细胞募集至肾小球，从而参与糖尿病肾病的发病机制。本研究旨在检测1型糖尿病患者血清MCP-1浓度、单核细胞Mac-1和淋巴细胞功能相关抗原-1（LFA-1）表达与肾病之间的潜在关联。
将51例有或无糖尿病肾病的1型糖尿病患者的血清MCP-1水平及单核细胞黏附分子表达与15例匹配的健康对照者进行比较。采用酶联免疫吸附测定法测定血清MCP-1浓度，通过流式细胞术检测单核细胞黏附分子Mac-1和LFA-1的表达。
与对照组相比，糖尿病患者血清MCP-1水平及Mac-1表达显著升高，但LFA-1表达无明显变化。对照组患者血清MCP-1平均水平为137.2±71.4 pg/mL，而糖尿病患者为246.2±114.9 pg/mL（p = 0.002）。血清MCP-1水平与糖化血红蛋白（HbA1c）及空腹血糖水平呈正相关。有或无糖尿病肾病的1型糖尿病患者血清MCP-1水平及单核细胞黏附分子表达无差异。
在1型糖尿病患者中，循环MCP-1浓度及Mac-1表达水平主要受血糖控制影响，而非糖尿病肾病的存在。
