Cao Jin, Murch Susan J, O'Brien Robert, Saxena Praveen K
Chemistry, I.K. Barber School of Arts and Sciences, University of British Columbia Okanagan, 3333 University Way, Kelowna V1V 1V7, Canada.
J Chromatogr A. 2006 Nov 17;1134(1-2):333-7. doi: 10.1016/j.chroma.2006.09.079.
Currently, the information available on the physiological functions of melatonin in higher plants is rather limited and the role of plant melatonin in human health remains undetermined. Research in this area has been slow due to lack of efficient analytical methods for rapid identification and quantification of the melatonin and related compounds in complex plant matrices. In this communication, we report the development of a rapid, accurate method for extraction, detection and quantification of plant melatonin, serotonin and indole-3-acetic acid (IAA) by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), and atmospheric pressure photoionization (APPI), respectively. The limit of detection (LOD) of melatonin in the plant extraction was 5 pg/ml and the limit of quantification (LOQ) was 0.02 ng/ml, as well as LOD for serotonin was 100 pg/ml and the LOQ was 5 ng/ml, LOD for IAA was 50 pg/ml and the LOQ was 0.7 ng/ml. There was a linear relationship between melatonin, serotonin, and IAA concentration and peak area over a quantifiable range of 0.02 ng/ml to 0.1 mg/ml, 5 ng/ml to 0.1 mg/ml, and 0.7 ng/ml to 0.1 mg/ml, respectively, in the plant extract.
目前，关于褪黑素在高等植物中的生理功能的现有信息相当有限，植物褪黑素在人类健康中的作用仍未确定。由于缺乏用于快速鉴定和定量复杂植物基质中褪黑素及相关化合物的有效分析方法，该领域的研究进展缓慢。在本通讯中，我们报告了一种分别采用电喷雾电离（ESI）、大气压化学电离（APCI）和大气压光电离（APPI）的液相色谱-串联质谱（LC-MS/MS）技术，用于快速、准确地提取、检测和定量植物褪黑素、血清素和吲哚-3-乙酸（IAA）的方法。植物提取物中褪黑素的检测限（LOD）为5 pg/ml，定量限（LOQ）为0.02 ng/ml；血清素的LOD为100 pg/ml，LOQ为5 ng/ml；IAA的LOD为50 pg/ml，LOQ为0.7 ng/ml。在植物提取物中，褪黑素、血清素和IAA浓度与峰面积在0.02 ng/ml至0.1 mg/ml、5 ng/ml至0.1 mg/ml和0.7 ng/ml至0.1 mg/ml的可定量范围内分别呈线性关系。
