Aoyagi Shuichi, Onishi Hiraku, Machida Yoshiharu
Department of Drug Delivery Research, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan.
Int J Pharm. 2007 Feb 7;330(1-2):138-45. doi: 10.1016/j.ijpharm.2006.09.016. Epub 2006 Sep 17.
Novel wound dressings composed of chitosan (CH) film and minocycline hydrochloride (MH) were prepared using commercial polyurethane film (Tegaderm) as a backing. CHs with deacetylation degrees of 67%, 83% and 96% (mol/mol), named CH67, CH83 and CH96, respectively, were used. Wound dressing with a large piece of Tegaderm film (4 cm x 4 cm), named CH-MH-N, and wound dressing prepared by cutting CH-MH-N to the wound size, named CH-MH-A, were developed. As CH67-MH-N and CH83-MH-N showed the sustained release of minocycline in vitro, CH67 and CH83 were used as chitosan in the in vivo studies. Various formulations were applied to severe burn wounds in rats in the early stage, and the wound status and change in the wound surface area were examined. The use of 10mg of MH and complete sealing with Tegaderm had a negative effect. MH ointment was not effective, but Geben cream was fairly effective. However, CH83-MH-A containing 2mg of MH (CH83-MH2-A) and CH83 film showed an excellent effect. Considering the elimination of pus, CH83-MH2-A tended to be better than CH83 film. CH83-MH2-A is suggested as a useful formulation for the treatment of severe burn wounds.
以商用聚氨酯薄膜（德莫林）为背衬，制备了由壳聚糖（CH）薄膜和盐酸米诺环素（MH）组成的新型伤口敷料。使用了脱乙酰度分别为67%、83%和96%（摩尔/摩尔）的壳聚糖，分别命名为CH67、CH83和CH96。开发了一种使用大片德莫林薄膜（4厘米×4厘米）的伤口敷料，命名为CH-MH-N，以及通过将CH-MH-N裁剪成伤口大小而制备的伤口敷料，命名为CH-MH-A。由于CH67-MH-N和CH83-MH-N在体外显示出米诺环素的持续释放，因此在体内研究中使用CH67和CH83作为壳聚糖。在大鼠严重烧伤伤口的早期应用了各种制剂，并检查了伤口状况和伤口表面积的变化。使用10毫克的MH并用德莫林完全密封有负面影响。MH软膏无效，但格本乳膏相当有效。然而，含有2毫克MH的CH83-MH-A（CH83-MH2-A）和CH83薄膜显示出优异的效果。考虑到脓液的清除，CH83-MH2-A往往比CH83薄膜更好。CH83-MH2-A被认为是治疗严重烧伤伤口的一种有用制剂。
