单克隆免疫荧光法与银染色法在检测卡氏肺孢子虫肺炎中的比较
Monoclonal immunofluorescence compared with silver stain for investigating Pneumocystis carinii pneumonia.
作者信息
Midgley J, Parsons P A, Shanson D C, Husain O A, Francis N
机构信息
Department of Microbiology, St Stephen's Hospital, London.
出版信息
J Clin Pathol. 1991 Jan;44(1):75-6. doi: 10.1136/jcp.44.1.75.
Abstract
Two hundred and eighty two specimens from 220 patients positive for HIV with respiratory tract symptoms, or febrile illness, or both, were examined for the presence of Pneumocystis carinii. Specimens were either induced sputum samples or bronchoalveolar lavage fluids. To establish the optimal method for laboratory diagnosis a comparison was made of detection of the organism by use of monoclonal antibody and immunofluorescence with conventional silver staining methods. Three commercially available reagents for immunofluorescence were also compared. Immunofluorescence was significantly more sensitive than the silver stain and the best results for immunofluorescence were obtained using. Northumbria Biologicals Ltd reagents.
摘要
对220例出现呼吸道症状、发热疾病或两者皆有的HIV阳性患者的282份标本进行了卡氏肺孢子虫检测。标本为诱导痰样本或支气管肺泡灌洗液。为确定实验室诊断的最佳方法，将使用单克隆抗体和免疫荧光检测该病原体的方法与传统银染色方法进行了比较。还比较了三种市售免疫荧光试剂。免疫荧光法比银染色法明显更敏感，使用诺森比亚生物制品有限公司的试剂获得了最佳免疫荧光检测结果。
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