反刍动物立克次氏体抗原蛋白的鉴定
Identification of the antigenic proteins of Cowdria ruminantium.
作者信息
Rossouw M, Neitz A W, de Waal D T, du Plessis J L, van Gas L, Brett S
机构信息
Veterinary Research Institute, Onderstepoort.
出版信息
Onderstepoort J Vet Res. 1990 Dec;57(4):215-21.
Immunoblotting of Cowdria ruminantium proteins with sheep or bovine antiserum identified 2 antigenically conserved proteins, one being an immunodominant 31 kDa and the other a minor 27 kDa protein. These proteins are present in the electrophoretic profiles of the Welgevonden, Ball 3 and Kwanyanga stocks and are recognized by sheep antiserum to the Welgevonden, Ball 3, Kwanyanga, Mali, Comoro, Breed, Germishuys, Kümm and Mara stocks and by bovine antiserum to the Welgevonden stock of C. ruminantium. The stocks did not reveal identical or unique antigenic properties which could explain differences in pathogenicity and cross-immunity observed amongst the various stocks of C. ruminantium.
用绵羊或牛抗血清对反刍兽考德里氏体蛋白进行免疫印迹分析，鉴定出2种抗原保守蛋白，一种是免疫显性的31 kDa蛋白，另一种是次要的27 kDa蛋白。这些蛋白存在于韦尔赫芬登、鲍尔3号和夸扬加毒株的电泳图谱中，能被针对韦尔赫芬登、鲍尔3号、夸扬加、马里、科摩罗、布里德、格米舒伊斯、库姆和马拉毒株的绵羊抗血清以及针对反刍兽考德里氏体韦尔赫芬登毒株的牛抗血清识别。这些毒株并未显示出相同或独特的抗原特性，而这些特性本可解释在反刍兽考德里氏体不同毒株间观察到的致病性差异和交叉免疫情况。
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