用于检测鼠白血病病毒的脾细胞空斑试验。
Splenocyte plaque assay for the detection of murine leukemia virus.
作者信息
Melief C J, Datta S K, Louie S, Johnson S, Melief M, Schwartz R S
出版信息
Proc Soc Exp Biol Med. 1975 Sep;149(4):1015-8. doi: 10.3181/00379727-149-38946.
PMID:170622
Abstract
A modified XC assay for murine leukemia virus (MuLV) employing splenocytes taken directly from the animal is described. This modification can be more than 1000 times more sensitive than XC plaque assays employing tissue extracts. This technique should lend itself readily to the quantitation of infectious MuLV in defined populations of lymphoid cells.
摘要
描述了一种改良的用于小鼠白血病病毒（MuLV）的XC试验，该试验使用直接取自动物的脾细胞。这种改良比使用组织提取物的XC空斑试验灵敏度高1000倍以上。该技术应易于用于定量特定淋巴细胞群体中感染性MuLV的含量。
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