Green N, Hiai H, Elder J H, Schwartz R S, Khiroya R H, Thomas C Y, Tsichlis P N, Coffin J M
J Exp Med. 1980 Aug 1;152(2):249-64. doi: 10.1084/jem.152.2.249.
HRS/J inbred mice carry a mutant autosomal recessive gene (hr), which in homozygotes coincides with susceptibility to spontaneous thymic leukemia. Unlike their heterozygote (hr/+) littermates, hr/hr homozygotes express high levels of xenotropic virus during the preleukemic period, and viruses with a broadened host range (termed polytropic viruses) can be isolated from their preleukemic and leukemic tissues. Because hr/hr and hr/+ mice are otherwise genetically identical, the virological differences between them support the role of polytropic viruses in the generation of thymic leukemia. In the present report we show that the HRS/J polytropic viruses are env gene recombinants with unique oligonucleotide and peptide maps. These polytropic viruses appear to arise by recombination between ecotropic virus and an unidentified genome related, but not identical to, the endogenous xenotropic viruses. Moreover, polytropic viruses not only accelerate leukemogenesis in HRS/J mice, but also induce thymic leukemia in the low leukemia strain CBA/J. By contrast, cloned ecotropic and xenotropic viruses have no leukemogenic action.
HRS/J近交系小鼠携带一个突变的常染色体隐性基因（hr），该基因在纯合子中与自发性胸腺白血病易感性相关。与它们的杂合子（hr/+）同窝小鼠不同，hr/hr纯合子在白血病前期表达高水平的嗜异性病毒，并且可以从它们的白血病前期和白血病组织中分离出具有更广泛宿主范围的病毒（称为多嗜性病毒）。由于hr/hr和hr/+小鼠在其他方面基因相同，它们之间的病毒学差异支持多嗜性病毒在胸腺白血病发生中的作用。在本报告中，我们表明HRS/J多嗜性病毒是具有独特寡核苷酸和肽图谱的env基因重组体。这些多嗜性病毒似乎是由嗜亲性病毒与一个未鉴定的基因组之间重组产生的，该基因组与内源性嗜异性病毒相关但不相同。此外，多嗜性病毒不仅加速HRS/J小鼠的白血病发生，还能在低白血病发生率的CBA/J品系中诱导胸腺白血病。相比之下，克隆的嗜亲性病毒和嗜异性病毒没有致白血病作用。
