Raynes J G, Eagling S, McAdam K P
Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, England.
Clin Exp Immunol. 1991 Mar;83(3):488-91. doi: 10.1111/j.1365-2249.1991.tb05666.x.
Interleukin-6 (IL-6, BSF-2 or IFN-beta 2) is thought to be the major regulator of the acute-phase protein response that follows tissue injury and inflammation, with interleukin-1 (IL-1), tumour necrosis factor and more recently, LIF or HSF III, slightly stimulatory on only certain acute phase proteins. The synthesis of the major acute-phase protein SAA, originally described as being synthesized in response to IL-1, has been claimed recently to be mainly under IL-6 regulation. Our results show that in the human hepatoma cell line HuH-7, IL-1 is the major stimulating cytokine increasing SAA synthesis by a factor in excess of 100-fold. We also show that under most conditions interleukin-6 and tumour necrosis factor stimulate additively in combination with IL-1. Isoelectric focusing has demonstrated that SAA1 and SAA2 alpha are expressed but not SAA2 beta. The HuH-7 cell line is IL-6 responsive since haptoglobin is stimulated mainly by IL-6.
白细胞介素-6（IL-6，即BSF-2或IFN-β2）被认为是组织损伤和炎症后急性期蛋白反应的主要调节因子，白细胞介素-1（IL-1）、肿瘤坏死因子，以及最近发现的白血病抑制因子（LIF）或热休克因子III（HSF III），仅对某些急性期蛋白有轻微刺激作用。主要急性期蛋白血清淀粉样蛋白A（SAA）最初被描述为在IL-1作用下合成，最近有人声称其合成主要受IL-6调控。我们的结果表明，在人肝癌细胞系HuH-7中，IL-1是增加SAA合成的主要刺激细胞因子，其增加幅度超过100倍。我们还表明，在大多数情况下，白细胞介素-6和肿瘤坏死因子与IL-1联合刺激具有相加作用。等电聚焦显示表达了SAA1和SAA2α，但未表达SAA2β。HuH-7细胞系对IL-6有反应，因为触珠蛋白主要受IL-6刺激。
