Pereira Maria Angélica G, Ortiz-Agostinho Carmen L, Nishitokukado Iêda, Sato Maria-N, Damião Adérson O M C, Alencar Marília L, Abrantes-Lemos Clarice P, Cançado Eduardo L R, de Brito Thales, Ioshii Sérgio O, Valarini Sandra B M, Sipahi Aytan M
Department of Gastroenterology, Medical Investigation Laboratories (LIM 07), University of São Paulo School of Medicine, São Paulo, SP, Brazil.
World J Gastroenterol. 2006 Oct 28;12(40):6546-50. doi: 10.3748/wjg.v12.i40.6546.
To determine the prevalence of celiac disease in a group of volunteer blood donors at a blood bank in the city of Curitiba, Brazil through detection of the serum marker immunoglobulin A (IgA) antitransglutaminase antibody.
Blood samples collected from 2086 healthy subjects at the Paraná State Center for Hematology and Hemotherapy in Curitiba were submitted to ELISA testing for the IgA antitransglutaminase antibody. Positive samples received IgA antiendomysium antibody test through indirect immunofluorescence using human umbilical cord as substrate. Subsequently, patients who were positive on both tests underwent small bowel (distal duodenum) biopsy.
Six subjects, four males and two females, tested positive for the two serum markers. Five of the six were submitted to intestinal biopsy (one declined the procedure). Biopsy results revealed changes in the distal duodenum mucosa (three classified as Marsh IIIb lesions and two as Marsh II lesions). Most donors diagnosed having celiac disease presented multiple symptoms (gastrointestinal tract complaints). One donor reported having a family history of celiac disease (in a niece).
Among apparently healthy blood donors, the prevalence of biopsy-confirmed celiac disease was approximately 1:417, similar to that seen in European countries.
通过检测血清标志物免疫球蛋白A（IgA）抗转谷氨酰胺酶抗体，确定巴西库里蒂巴市一家血库中一组志愿献血者的乳糜泻患病率。
从库里蒂巴巴拉那州血液学和血液疗法中心的2086名健康受试者采集血样，进行IgA抗转谷氨酰胺酶抗体的酶联免疫吸附测定（ELISA）检测。阳性样本通过以人脐带为底物的间接免疫荧光法接受IgA抗肌内膜抗体检测。随后，两项检测均呈阳性的患者接受小肠（十二指肠远端）活检。
6名受试者（4名男性和2名女性）的两种血清标志物检测呈阳性。6人中的5人接受了肠道活检（1人拒绝该检查）。活检结果显示十二指肠远端黏膜有变化（3例分类为马什IIIb型病变，2例为马什II型病变）。大多数被诊断患有乳糜泻的献血者有多种症状（胃肠道不适）。1名献血者报告有乳糜泻家族史（在一个侄女身上）。
在表面健康的献血者中，经活检确诊的乳糜泻患病率约为1:417，与欧洲国家的患病率相似。
