Owen William E, Martins Thomas B, Litwin Christine M, Roberts William L
ARUP Institute for Clinical and Experimental Pathology, University of Utah, Health Sciences Center, Salt Lake City 84108, USA.
Am J Clin Pathol. 2006 Dec;126(6):900-5. doi: 10.1309/KUA926D3YAPFYQG8.
TORCH is an acronym for Toxoplasma gondii (Toxo), other microorganisms (eg, syphilis), rubella virus (RV), cytomegalovirus (CMV), and herpes simplex virus (HSV) that are associated with congenital abnormalities from maternal infection. We evaluated linearity, imprecision, and comparison with commercially available methods of the IMMULITE 2000 (Diagnostic Products, Los Angeles, CA) Toxo IgG, Toxo IgM, RV IgG, RV IgM, CMV IgG, and HSV IgG assays. Linearity and imprecision results were acceptable. The IMMULITE 2000 assays show good concordance with other commercially available methods except for Toxo IgM and RV IgM. Toxo IgM showed better concordance with a consensus of 3 of 4 (Access, Beckman Coulter, Fullerton, CA; IMMULITE 2000; Platelia, Bio-Rad Laboratories Diagnostics Group, Redmond, WA; and Vidas, bioMerieux, Hazelwood, MO) assays than with Access alone. The RV IgM assay showed better concordance with the Zeus method than with the Diamedix method (Diamedix, Miami, FL). The IMMULITE 2000 TORCH assays studied show acceptable performance and are suitable for routine clinical use. Some commercial assays for Toxo IgM and RV IgM show rather poor concordance.
TORCH是弓形虫（Toxo）、其他微生物（如梅毒）、风疹病毒（RV）、巨细胞病毒（CMV）和单纯疱疹病毒（HSV）的首字母缩写，这些病原体与母体感染所致的先天性异常有关。我们评估了IMMULITE 2000（诊断产品公司，加利福尼亚州洛杉矶）弓形虫IgG、弓形虫IgM、风疹病毒IgG、风疹病毒IgM、巨细胞病毒IgG和单纯疱疹病毒IgG检测方法的线性、不精密度，并与市售方法进行了比较。线性和不精密度结果可以接受。除弓形虫IgM和风疹病毒IgM外，IMMULITE 2000检测方法与其他市售方法显示出良好的一致性。弓形虫IgM与4种检测方法中的3种（Access，贝克曼库尔特公司，加利福尼亚州富勒顿；IMMULITE 2000；Platelia，伯乐生命医学产品公司诊断部，华盛顿州雷德蒙德；以及Vidas，生物梅里埃公司，密苏里州黑兹尔伍德）的一致性，优于仅与Access检测方法的一致性。风疹病毒IgM检测方法与Zeus方法的一致性，优于与Diamedix方法（Diamedix，佛罗里达州迈阿密）的一致性。所研究的IMMULITE 2000 TORCH检测方法表现可接受，适用于常规临床应用。一些市售的弓形虫IgM和风疹病毒IgM检测方法显示出相当差的一致性。
