Harada Y
Acta Pathol Jpn. 1975 Jul;25(4):451-61. doi: 10.1111/j.1440-1827.1975.tb00865.x.
Induction of a spontaneously metastasizing carcinoma in rats was attempted. Four-week-old Sprague-Dawley female rats were thymectomized or/and splenectomized and fed 200 mg (20 mg times 10) of 3-methylcholanthrene from 7 weeks of age. In addition to these treatments, the early-appearing tumors were excised in order to select by isoimmunity the late-appearing ones that were less antigenic. The latter were easily transplanted into normal syngeneic female rats with metastasis to remote organs. This metastasizing capacity of the tumor became an inherent character in syngeneic normal rats from generation to generation of transplantation. With one of these tumors (MRMT-1) many cancer cells were histologically detected in circulating blood 3 days after tumor transplantation and arrested in capillary beds of lungs. The spontaneous metastasis to lymph nodes and lungs was macroscopically found within several weeks after tumor transplantation.
尝试在大鼠中诱导自发转移癌。对4周龄的斯普拉格-道利雌性大鼠进行胸腺切除或/和脾切除，并从7周龄开始喂食200毫克（20毫克×10）的3-甲基胆蒽。除了这些处理外，切除早期出现的肿瘤，以便通过同种免疫选择抗原性较低的晚期出现的肿瘤。后者很容易移植到同基因正常雌性大鼠体内，并转移到远处器官。肿瘤的这种转移能力在同基因正常大鼠的一代又一代移植中成为一种固有特征。在肿瘤移植后3天，用其中一种肿瘤（MRMT-1）在循环血液中通过组织学检测到许多癌细胞，并在肺毛细血管床中停滞。在肿瘤移植后几周内，肉眼可见肿瘤自发转移至淋巴结和肺。
