Mazumdar Saikat Datta, Hartmann Markus, Kämpfer Peter, Keusgen Michael
Institute for Pharmaceutical Chemistry, Philipps-Universität Marburg, Marbacher Weg 6, D-35032 Marburg, Germany.
Biosens Bioelectron. 2007 Apr 15;22(9-10):2040-6. doi: 10.1016/j.bios.2006.09.004. Epub 2006 Oct 31.
The Plasmonic surface plasmon resonance (SPR) device was used to develop a rapid, simple and specific immunoassay for detection of Salmonella in milk. Rapid detection of Salmonella contamination is a major challenge for the food industry. Salmonella contamination is well known in all foods including pasteurised milk. The SPR assay was developed as a sandwich model using a polyclonal antibody against Salmonella as capture and detection antibody. Milk spiked with Salmonella typhimurium cells, killed by thimerosal (1%, w/w) treatment was used. Using the Plasmonic SPR assay it was possible to detect S. typhimurium down to a concentration of 1.25 x 10(5) cells ml(-1) in both milk and buffer system. The results obtained are comparable with existing, approved rapid Salmonella detection techniques. No negative effects on the sensitivity of the assay are encountered due to the milk matrix. Hence, no sample preparation or clean-up steps are required. The sample volume requirement for the assay is only 10 microl. Using the assay S. typhimurium was detected in milk within 1h, whereas the cultural techniques require 3-4 days for presumptive positive isolates and further time for confirmation. The rapid tests require at least 24h for the results. The Plasmonic SPR device operates on the Kretschmann configuration and is a cuvette-based system with the advantage of having eight channels on one single SPR chip.
等离子体表面等离子体共振（SPR）装置被用于开发一种快速、简单且特异的免疫分析法，用于检测牛奶中的沙门氏菌。沙门氏菌污染的快速检测是食品工业面临的一项重大挑战。沙门氏菌污染在包括巴氏杀菌牛奶在内的所有食品中都广为人知。SPR分析是作为一种夹心模型开发的，使用抗沙门氏菌的多克隆抗体作为捕获抗体和检测抗体。使用经硫柳汞（1%，w/w）处理杀死的鼠伤寒沙门氏菌细胞加标的牛奶。使用等离子体SPR分析，在牛奶和缓冲液系统中都能够检测到低至1.25×10⁵个细胞/毫升浓度的鼠伤寒沙门氏菌。所获得的结果与现有的、经批准的沙门氏菌快速检测技术相当。牛奶基质对该分析的灵敏度没有负面影响。因此，无需样品制备或净化步骤。该分析所需的样品体积仅为10微升。使用该分析方法，在1小时内就能检测到牛奶中的鼠伤寒沙门氏菌，而培养技术对于初步阳性分离株需要3 - 4天，且进一步确认还需要更多时间。快速检测至少需要24小时才能得到结果。等离子体SPR装置采用Kretschmann结构，是一种基于比色皿的系统，优点是在一个SPR芯片上有八个通道。
