Mandu-Hrit Manuela, Haque Tasima, Lauzier Dominique, Kotsiopriftis Maria, Rauch Frank, Tabrizian Maryam, Henderson Janet E, Hamdy Reggie C
Shriners Hospital, Montreal Children Hospital, Division of Orthopaedics, McGill University, Montreal, Que., Canada.
Growth Factors. 2006 Sep;24(3):172-83. doi: 10.1080/08977190600621362.
Distraction osteogenesis (DO) is a surgical technique for generating new bone by applying controlled distraction of two bony segments post osteotomy. A limitation of the technique is the long time required for the new bone to consolidate. We investigated the effect of injecting osteogenic protein 1 (OP-1) at the beginning of distraction in a rabbit model of DO. Regenerate bone was evaluated using radiology, densitometry, micro-computed tomography (microCT) and histomorphometry. Immunohistochemsitry was used to evaluate changes in expression of various ligands, growth factors and receptors following OP-1 treatment. Compared to the control, a two-fold increase in bone volume was apparent for treated groups at 3 weeks post injection. An upregulation of almost all of the 41 genes examined was observed. Results suggested that applying OP-1 early during distraction can accelerate bone formation by the activation of numerous pathways. This study provides further insights on strategies to improve bone regeneration rate in DO.
牵张成骨术（DO）是一种通过在截骨术后对两个骨段施加可控牵张来生成新骨的外科技术。该技术的一个局限性是新骨巩固所需的时间较长。我们在兔牵张成骨模型中研究了在牵张开始时注射成骨蛋白1（OP-1）的效果。使用放射学、骨密度测定、微计算机断层扫描（microCT）和组织形态计量学对再生骨进行评估。免疫组织化学用于评估OP-1治疗后各种配体、生长因子和受体表达的变化。与对照组相比，注射后3周时治疗组的骨体积明显增加了两倍。观察到所检测的41个基因中几乎所有基因均上调。结果表明，在牵张早期应用OP-1可通过激活众多途径加速骨形成。本研究为提高牵张成骨术中骨再生率的策略提供了进一步的见解。
