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Purification of recombinant proteins from Escherichia coli at low expression levels by inverse transition cycling.

作者信息

Christensen Trine, Trabbic-Carlson Kimberly, Liu Wenge, Chilkoti Ashutosh

机构信息

Department of Biomedical Engineering, Box 90281, Duke University, Durham, NC 27708, USA.

出版信息

Anal Biochem. 2007 Jan 1;360(1):166-8. doi: 10.1016/j.ab.2006.09.020. Epub 2006 Nov 3.

DOI:10.1016/j.ab.2006.09.020
PMID:17084802
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1831849/
Abstract
摘要

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本文引用的文献

1
One-pot glyco-affinity precipitation purification for enhanced proteomics: the flexible alignment of solution-phase capture/release and solid-phase separation.
J Proteome Res. 2005 Nov-Dec;4(6):2355-9. doi: 10.1021/pr050245g.
2
Self-cleavable stimulus responsive tags for protein purification without chromatography.用于无需色谱法的蛋白质纯化的自切割刺激响应标签。
J Am Chem Soc. 2005 Aug 17;127(32):11228-9. doi: 10.1021/ja0531125.
3
Simple bioseparations using self-cleaving elastin-like polypeptide tags.使用自切割弹性蛋白样多肽标签的简单生物分离方法。
Nat Methods. 2005 Sep;2(9):659-61. doi: 10.1038/nmeth787.
4
Genetically engineered elastin-protein A fusion as a universal platform for homogeneous, phase-separation immunoassay.基因工程改造的弹性蛋白-蛋白A融合体作为用于均相、相分离免疫测定的通用平台。
Anal Chem. 2005 Apr 15;77(8):2318-22. doi: 10.1021/ac0484326.
5
Comparison of affinity tags for protein purification.用于蛋白质纯化的亲和标签比较。
Protein Expr Purif. 2005 May;41(1):98-105. doi: 10.1016/j.pep.2005.01.019.
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Temperature-triggered purification of antibodies.温度触发的抗体纯化
Biotechnol Bioeng. 2005 May 5;90(3):373-9. doi: 10.1002/bit.20451.
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Informatics for protein identification by mass spectrometry.用于通过质谱法进行蛋白质鉴定的信息学。
Methods. 2005 Mar;35(3):223-36. doi: 10.1016/j.ymeth.2004.08.014. Epub 2005 Jan 13.
8
Expression and purification of recombinant proteins from Escherichia coli: Comparison of an elastin-like polypeptide fusion with an oligohistidine fusion.大肠杆菌重组蛋白的表达与纯化：弹性蛋白样多肽融合与寡聚组氨酸融合的比较
Protein Sci. 2004 Dec;13(12):3274-84. doi: 10.1110/ps.04931604.
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Quantification of the effects of chain length and concentration on the thermal behavior of elastin-like polypeptides.链长和浓度对类弹性蛋白多肽热行为影响的量化
Biomacromolecules. 2004 May-Jun;5(3):846-51. doi: 10.1021/bm034215n.
10
Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity?蛋白质融合对环境响应性类弹性蛋白多肽转变温度的影响：表面疏水性的作用？
Protein Eng Des Sel. 2004 Jan;17(1):57-66. doi: 10.1093/protein/gzh006.