Davis W C, Splitter G S
Department of Veterinary Microbiology/Pathology, Washington State University, Pullman 99164-7040.
Vet Immunol Immunopathol. 1991 Jan;27(1-3):43-50. doi: 10.1016/0165-2427(91)90077-p.
The data obtained in the workshop provide further evidence that CH128A and IL-A26 and the 12 new mAbs that form a cluster recognise the bovine orthologue of CD2. The mAbs inhibit rosetting with SRBC, stain cells in primary and secondary lymphoid organs in patterns consistent with those obtained in humans with anti-CD2 mAbs, and the 11 IgG mAbs all immunoprecipitate a peptide with a Mr of 58-62 kDa. It is not clear from the studies whether the epitopes defined by the mAbs correspond with the region I and II epitopes present on CD2. None of the data suggest that any of the mAbs recognise the region III (CDD2R) epitope (Peterson and Seed, 1987; Knapp et al., 1989). Further studies are now needed to define the physical and functional relation of the epitopes and establish whether antibody-mediated activation corresponds with that noted in humans. Data reported in one study (Baldwin et al., 1988) with IL-A26 suggest possible differences in the requirements for activation. In addition, further studies are needed to demonstrate how many cell types express BoCD2. In mice, evidence has been presented which shows the mouse orthologue is expressed on some B cells (Yagitta et al., 1989). Studies in cattle have clearly shown CD2 is present on the majority of CD4+ and CD8+ T-cells and a small population of CD4-/CD8- cells (Baldwin et al., 1988; Davis, unpublished observations). Evidence presented in this workshop has shown that some CD2+ cells express a WC2 molecule (Sopp et al., 1991).(ABSTRACT TRUNCATED AT 250 WORDS)
在研讨会上获得的数据进一步证明，CH128A、IL-A26以及形成一个簇的12种新单克隆抗体识别牛的CD2直系同源物。这些单克隆抗体抑制与SRBC的玫瑰花结形成，以与人类抗CD2单克隆抗体所获模式一致的方式对初级和次级淋巴器官中的细胞进行染色，并且所有11种IgG单克隆抗体均免疫沉淀出一条分子量为58 - 62 kDa的肽段。从这些研究中尚不清楚单克隆抗体所定义的表位是否与CD2上存在的I区和II区表位相对应。没有任何数据表明任何一种单克隆抗体识别III区（CDD2R）表位（彼得森和西得，1987年；克纳普等人，1989年）。现在需要进一步研究来确定这些表位的物理和功能关系，并确定抗体介导的激活是否与人类中所观察到的一致。一项关于IL-A26的研究（鲍德温等人，1988年）报告的数据表明激活要求可能存在差异。此外，需要进一步研究以证明有多少细胞类型表达牛CD2。在小鼠中，已有证据表明小鼠直系同源物在一些B细胞上表达（矢田等人，1989年）。对牛的研究清楚地表明CD2存在于大多数CD4 +和CD8 + T细胞以及一小部分CD4 - /CD8 -细胞上（鲍德温等人，1988年；戴维斯，未发表的观察结果）。本次研讨会提供的证据表明一些CD2 +细胞表达WC2分子（索普等人，1991年）。（摘要截短至250字）
