Chen Fang, Lu Jin-Chun, Xu Hui-Ru, Huang Yu-Feng, Lu Nian-Qing
Department of Reproduction and Genetics, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, PR China.
Clin Chem Lab Med. 2006;44(11):1335-9. doi: 10.1515/CCLM.2006.253.
Few reports of the effects of treatment with chymotrypsin on the determination of sperm parameters and seminal biochemistry markers are documented.
Sperm parameters of 63 liquefied and 27 non-liquefied samples, untreated or treated with chymotrypsin, were evaluated using computer-assisted semen analysis. In addition, biochemistry markers such as gamma-glutamyltranspeptidase, alpha-glucosidase and fructose in 50 liquefied and 39 non-liquefied samples, untreated or treated with chymotrypsin, were determined.
Treatment with chymotrypsin had no effect on sperm concentration, motility, motility a and b, straightness, curvilinear velocity, straight line velocity, average path velocity and beat cross frequency in both liquefied and non-liquefied semen. However, linearity (p=0.025) decreased and the amplitude of the lateral head (p=0.029) increased significantly in non-liquefied semen after treatment with chymotrypsin. The levels of gamma-glutamyltranspeptidase, alpha-glucosidase and fructose in seminal plasma were unaffected by chymotrypsin, regardless of liquefaction status.
Chymotrypsin had no effects on the detection of sperm parameters and biochemistry markers, and could be used to treat non-liquefied samples before semen analysis in the andrology laboratory.
关于胰凝乳蛋白酶治疗对精子参数测定及精液生化标志物影响的报道较少。
使用计算机辅助精液分析评估63份液化样本和27份未液化样本（未处理或经胰凝乳蛋白酶处理）的精子参数。此外，测定50份液化样本和39份未液化样本（未处理或经胰凝乳蛋白酶处理）中的γ-谷氨酰转肽酶、α-葡萄糖苷酶和果糖等生化标志物。
胰凝乳蛋白酶处理对液化和未液化精液的精子浓度、活力、a级和b级活力、直线性、曲线速度、直线速度、平均路径速度及鞭打交叉频率均无影响。然而，胰凝乳蛋白酶处理后，未液化精液的线性度（p = 0.025）降低，头部侧向幅度（p = 0.029）显著增加。无论液化状态如何，精浆中γ-谷氨酰转肽酶、α-葡萄糖苷酶和果糖水平均不受胰凝乳蛋白酶影响。
胰凝乳蛋白酶对精子参数和生化标志物检测无影响，可用于男科实验室精液分析前处理未液化样本。
