Gao De, Agayan Rodney R, Xu Hao, Philbert Martin A, Kopelman Raoul
Department of Chemistry, University of Michigan, Ann Arbor, Michigan 48109-1055, USA.
Nano Lett. 2006 Nov;6(11):2383-6. doi: 10.1021/nl0617179.
We describe here a nontoxic two-photon photodynamic nanoparticle platform and its cellular application. We demonstrate that the dye's potential toxicity can be circumvented by its permanent encapsulation into a biocompatible nanoparticle polymer matrix; this was examined by dye leaching experiments and confirmed by cell uptake experiments. Infrared two-photon nanoplatform phototoxicity was demonstrated for rat C6 glioma cells, while the controls showed no dark toxicity for these living cells.
我们在此描述一种无毒的双光子光动力纳米颗粒平台及其细胞应用。我们证明,通过将染料永久封装在生物相容性纳米颗粒聚合物基质中,可以规避染料的潜在毒性;这通过染料浸出实验进行了检测,并通过细胞摄取实验得到了证实。已证明红外双光子纳米平台对大鼠C6胶质瘤细胞具有光毒性,而对照组对这些活细胞没有暗毒性。