一项关于酵母醇脱氢酶催化烟酰胺腺嘌呤二核苷酸氧化正丁醇和丙醇-2的研究。
A study of the oxidation of butan-1-ol and propan-2-ol by nicotinamide-adenine dinucleotide catalysed by yeast alcohol dehydrogenase.
作者信息
Dickenson C J, Dickinson F M
出版信息
Biochem J. 1975 Jun;147(3):541-7. doi: 10.1042/bj1470541.
Abstract
- The kinetics of oxidation of butan-1-ol and propan-2-ol by NAD+, catalysed by yeast alcohol dehydrogenase, were studied at 25 degrees C from pH 5.5 to 10, and at pH 7.05 from 14 degrees to 44 degrees C, 2. Under all conditions studied the results are consistent with a mechanism whereby some dissociation of coenzyme from the active enzyme-NAD+-alcohol ternary complexes occurs, and the mechanism is therefore not strictly compulsory order. 3. A primary 2H isotopic effect on the maximum rates of oxidation of [1-2H2]butan-1-ol and [2H7]propan-2-ol was found at 25 degrees C over the pH range 5.5-10. Further, in stopped-flow experiments at pH 7.05 and 25 degrees C, there was no transient formation of NADH in the oxidation of butan-1-ol and propan-2-ol. The principal rate-limiting step in the oxidation of dependence on pH of the maximum rates of oxidation of butan-1-ol and propan-2-ol is consisten with the possibility that histidine and cysteine residues may affect or control catalysis.
摘要
- 在25℃下，pH值从5.5到10，以及在pH 7.05、温度从14℃到44℃的条件下，研究了酵母醇脱氢酶催化的NAD⁺氧化正丁醇和2-丙醇的动力学。2. 在所有研究条件下，结果都符合一种机制，即辅酶从活性酶-NAD⁺-醇三元复合物中发生某种解离，因此该机制并非严格的强制顺序。3. 在25℃、pH范围5.5 - 10内，发现了[1-²H₂]正丁醇和[²H₇]2-丙醇氧化最大速率的一级²H同位素效应。此外，在pH 7.05和25℃的停流实验中，正丁醇和2-丙醇氧化过程中没有NADH的瞬态形成。正丁醇和2-丙醇氧化最大速率对pH的依赖性中，主要的限速步骤与组氨酸和半胱氨酸残基可能影响或控制催化作用的可能性一致。
相似文献
1
A study of the oxidation of butan-1-ol and propan-2-ol by nicotinamide-adenine dinucleotide catalysed by yeast alcohol dehydrogenase.一项关于酵母醇脱氢酶催化烟酰胺腺嘌呤二核苷酸氧化正丁醇和丙醇-2的研究。
Biochem J. 1975 Jun;147(3):541-7. doi: 10.1042/bj1470541.
2
A study of the kinetics and mechanism of yeast alcohol dehydrogenase with a variety of substrates.一项关于酵母乙醇脱氢酶与多种底物的动力学及作用机制的研究。
Biochem J. 1973 Feb;131(2):261-70. doi: 10.1042/bj1310261.
3
Estimation of rate and dissociation constants involving ternary complexes in reactions catalysed by yeast alcohol dehydrogenase.酵母乙醇脱氢酶催化反应中涉及三元复合物的速率常数和解离常数的估算。
Biochem J. 1978 Jun 1;171(3):629-37. doi: 10.1042/bj1710629.
4
The specificities and configurations of ternary complexes of yeast and liver alcohol dehydrogenases.酵母和肝脏乙醇脱氢酶三元复合物的特异性及构型
Biochem J. 1967 Jul;104(1):165-72. doi: 10.1042/bj1040165.
5
A study of the ionic properties of the essential histidine residue of yeast alcohol dehydrogenase in complexes of the enzyme with its coenzymes and substrates.关于酵母乙醇脱氢酶的必需组氨酸残基在该酶与其辅酶及底物复合物中的离子性质的研究。
Biochem J. 1977 Jan 1;161(1):73-82. doi: 10.1042/bj1610073.
6
The enthalpy change for the reduction of nicotinamide--adenine dinucleotide.烟酰胺腺嘌呤二核苷酸还原反应的焓变。
Biochem J. 1974 Nov;143(2):365-8. doi: 10.1042/bj1430365.
7
The kinetics and mechanism of liver alcohol dehydrogenase with primary and secondary alcohols as substrates.以伯醇和仲醇为底物时肝脏乙醇脱氢酶的动力学及作用机制
Biochem J. 1966 Jul;100(1):34-46. doi: 10.1042/bj1000034.
8
A study of the pH- and temperature-dependence of the reactions of yeast alcohol dehydrogenase with ethanol, acetaldehyde and butyraldehyde as substrates.一项关于酵母乙醇脱氢酶以乙醇、乙醛和丁醛为底物的反应对pH值和温度依赖性的研究。
Biochem J. 1975 May;147(2):303-11. doi: 10.1042/bj1470303.
9
Drosophila lebanonensis alcohol dehydrogenase: pH dependence of the kinetic coefficients.黎巴嫩果蝇乙醇脱氢酶：动力学系数的pH依赖性
Biochim Biophys Acta. 1999 Apr 12;1431(1):74-86. doi: 10.1016/s0167-4838(99)00028-x.
10
Purification and characterization of a nicotinamide adenine dinucleotide-dependent secondary alcohol dehydrogenase from Candida boidinii.来自博伊丁假丝酵母的烟酰胺腺嘌呤二核苷酸依赖性仲醇脱氢酶的纯化与特性分析
Biochim Biophys Acta. 1982 Jun 16;716(3):298-307. doi: 10.1016/0304-4165(82)90020-4.
引用本文的文献
1
Substitution of both histidines in the active site of yeast alcohol dehydrogenase 1 exposes underlying pH dependencies.取代酵母醇脱氢酶 1 活性部位的两个组氨酸会暴露出潜在的 pH 依赖性。
Chem Biol Interact. 2024 May 1;394:110992. doi: 10.1016/j.cbi.2024.110992. Epub 2024 Apr 4.
2
Solvent isotope and mutagenesis studies on the proton relay system in yeast alcohol dehydrogenase 1.酵母醇脱氢酶 1 中质子传递系统的溶剂同位素和诱变研究。
Chem Biol Interact. 2024 Jan 25;388:110853. doi: 10.1016/j.cbi.2023.110853. Epub 2023 Dec 25.
3
Specific base catalysis by yeast alcohol dehydrogenase I with substitutions of histidine-48 by glutamate or serine residues in the proton relay system.酵母醇脱氢酶 I 的组氨酸 48 突变为谷氨酸或丝氨酸残基，在质子传递系统中具有特定的碱基催化作用。
Chem Biol Interact. 2023 Sep 1;382:110558. doi: 10.1016/j.cbi.2023.110558. Epub 2023 May 27.
4
The Thr45Gly substitution in yeast alcohol dehydrogenase substantially decreases catalysis, alters pH dependencies, and disrupts the proton relay system.酵母醇脱氢酶中的 Thr45Gly 取代显著降低了催化活性，改变了 pH 依赖性，并破坏了质子传递系统。
Chem Biol Interact. 2021 Nov 1;349:109650. doi: 10.1016/j.cbi.2021.109650. Epub 2021 Sep 13.
5
Yeast alcohol dehydrogenase structure and catalysis.酵母醇脱氢酶的结构与催化。
Biochemistry. 2014 Sep 16;53(36):5791-803. doi: 10.1021/bi5006442. Epub 2014 Sep 3.
6
Activity of yeast alcohol dehydrogenases on benzyl alcohols and benzaldehydes: characterization of ADH1 from Saccharomyces carlsbergensis and transition state analysis.酵母醇脱氢酶对苄醇和苯甲醛的活性：嘉士伯酵母ADH1的特性及过渡态分析
Chem Biol Interact. 2009 Mar 16;178(1-3):16-23. doi: 10.1016/j.cbi.2008.10.037. Epub 2008 Nov 5.
7
Estimation of rate and dissociation constants involving ternary complexes in reactions catalysed by yeast alcohol dehydrogenase.酵母乙醇脱氢酶催化反应中涉及三元复合物的速率常数和解离常数的估算。
Biochem J. 1978 Jun 1;171(3):629-37. doi: 10.1042/bj1710629.
8
Inhibition by ethanol, acetaldehyde and trifluoroethanol of reactions catalysed by yeast and horse liver alcohol dehydrogenases.乙醇、乙醛和三氟乙醇对酵母和马肝醇脱氢酶催化反应的抑制作用。
Biochem J. 1978 Jun 1;171(3):613-27. doi: 10.1042/bj1710613.
9
The reactions of 1,10-phenanthroline with yeast alcohol dehydrogenase.1,10-菲咯啉与酵母乙醇脱氢酶的反应
Biochem J. 1977 Oct 1;167(1):237-44. doi: 10.1042/bj1670237.
10
Some properties of an alcohol dehydrogenase partially purified from baker's yeast grown without added zinc.从无添加锌培养的面包酵母中部分纯化得到的一种乙醇脱氢酶的某些特性。
Biochem J. 1976 Feb 1;153(2):309-19. doi: 10.1042/bj1530309.
本文引用的文献
1
EQUILIBRIUM REACTION RATES AND THE MECHANISMS OF LIVER AND YEAST ALCOHOL DEHYDROGENASE.平衡反应速率以及肝脏和酵母乙醇脱氢酶的作用机制
J Biol Chem. 1964 Nov;239:3908-14.
2
KINETIC STUDIES OF LIVER ALCOHOL DEHYDROGENASE AND PH EFFECTS WITH COENZYME PREPARATIONS OF HIGH PURITY.肝脏乙醇脱氢酶的动力学研究以及高纯度辅酶制剂的pH效应
J Biol Chem. 1963 Aug;238:2850-8.
3
The purification of nicotinamide adenine dinucleotide and kinetic effects of nucleotide impurities.烟酰胺腺嘌呤二核苷酸的纯化及核苷酸杂质的动力学效应
J Biol Chem. 1963 Apr;238:1538-43.
4
Kinetic studies of liver alcohol dehydrogenase.肝脏乙醇脱氢酶的动力学研究
Biochem J. 1962 Aug;84(2):244-54. doi: 10.1042/bj0840244.
5
6
The preparation and properties of crystalline alcohol dehydrogenase from liver.肝脏中结晶醇脱氢酶的制备及其性质
Biochem J. 1961 Aug;80(2):440-5. doi: 10.1042/bj0800440.
7
Apparatus for rapid and sensitive spectrophotometry.快速灵敏分光光度测定仪。
Biochem J. 1964 Apr;91(1):161-71. doi: 10.1042/bj0910161.
8
The binding of dihydronicotinamide--adenine dinucleotide and pyridine-3-aldehyde--adenine dinucleotide by yeast alcohol dehydrogenase.酵母乙醇脱氢酶对二氢烟酰胺-腺嘌呤二核苷酸和吡啶-3-醛-腺嘌呤二核苷酸的结合作用
Biochem J. 1970 Dec;120(4):821-30. doi: 10.1042/bj1200821.
9
Yeast alcohol dehydrogenase: SH groups, disulfide groups, quaternary structure, and reactivation by reductive cleavage of disulfide groups.酵母乙醇脱氢酶：巯基、二硫键、四级结构以及通过二硫键还原裂解实现的再激活作用
Eur J Biochem. 1969 Nov;11(1):73-9. doi: 10.1111/j.1432-1033.1969.tb00741.x.
10
Partial similarities between yeast and liver alcohol dehydrogenases.酵母乙醇脱氢酶与肝脏乙醇脱氢酶之间的部分相似性。
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2295-8. doi: 10.1073/pnas.70.8.2295.
Zotero 插件