Grössner-Schreiber Birte, Herzog Maren, Hedderich Jürgen, Dück Alexander, Hannig Matthias, Griepentrog Michael
Clinic of Restorative Dentistry and Periodontology, University of Kiel, Kiel, Germany.
Clin Oral Implants Res. 2006 Dec;17(6):736-45. doi: 10.1111/j.1600-0501.2006.01277.x.
A major consideration in designing dental implants is to create a surface that provides strong attachment of the implant to bone, connective tissue and epithelium. The aim of the present study was to examine the influence of different treatments of titanium (Ti) implant surfaces on focal adhesion contact (FAC) formation in fibroblast cultures. Human gingival fibroblasts were cultured on glass sheets and polished Ti discs with different surface coatings (applied by physical vapor deposition (PVD): Ti, titanium nitride (TiN), zirconium nitride (ZrN)) or on Ti discs with different surface topographies. For characterization of all surfaces, modified estimation of surface roughness and spacing parameter was carried out using a contact stylus profilometer. Contact angle measurements were carried out to calculate surface energy. Fibroblasts were prepared for transmission electron microscopy at day 3 after seeding, and the number of FACs and the ratio FAC/cellular cross-sections was determined at a length of 300 microm in ultrathin sections. To visualize the extracellular fibronectin and vitronectin molecules and the intracellular actin and vinculin in FAC areas, immunogold labeling was performed. The results revealed a strong correlation between the number of FACs and the surface roughness. The highest number of FACs and the majority of the immunogold-labeled intra- and extracellular matrix molecules were counted on surfaces with the lowest surface roughness: glass sheets coated with either Ti, TiN or ZrN (roughness average=0.03-0.1 microm). These surfaces appear to favor cellular attachment of human gingival fibroblasts and moreover in previous studies the hard coatings have been shown to reduce bacterial adhesion.
设计牙种植体时的一个主要考虑因素是创建一个能使种植体与骨、结缔组织和上皮牢固附着的表面。本研究的目的是检查钛（Ti）种植体表面的不同处理对成纤维细胞培养中粘着斑接触（FAC）形成的影响。将人牙龈成纤维细胞培养在玻璃板以及具有不同表面涂层（通过物理气相沉积（PVD）施加：Ti、氮化钛（TiN）、氮化锆（ZrN））的抛光Ti盘上，或者培养在具有不同表面形貌的Ti盘上。为了表征所有表面，使用接触式触针轮廓仪对表面粗糙度和间距参数进行了修正估计。进行接触角测量以计算表面能。在接种后第3天制备成纤维细胞用于透射电子显微镜检查，并在超薄切片中300微米的长度上确定FAC的数量以及FAC/细胞横截面的比率。为了可视化FAC区域中的细胞外纤连蛋白和玻连蛋白分子以及细胞内肌动蛋白和纽蛋白，进行了免疫金标记。结果显示FAC的数量与表面粗糙度之间存在很强的相关性。在表面粗糙度最低的表面上，即涂有Ti、TiN或ZrN的玻璃板（平均粗糙度 = 0.03 - 0.1微米）上，FAC的数量最多，并且免疫金标记的细胞内和细胞外基质分子的数量也最多。这些表面似乎有利于人牙龈成纤维细胞的细胞附着，而且在先前的研究中已表明硬涂层可减少细菌粘附。
