Groessner-Schreiber Birte, Neubert Anja, Müller Wolf-Dieter, Hopp Michael, Griepentrog Michael, Lange Klaus-Peter
University of Kiel, School of Dental Medicine, Clinic for Restorative Dentistry and Periodontology, Kiel, Germany.
J Biomed Mater Res A. 2003 Mar 15;64(4):591-9. doi: 10.1002/jbm.a.10417.
A major consideration in designing dental implants is the creation of a surface that provides strong attachment between the implant and bone, connective tissue, or epithelium. In addition, it is important to inhibit the adherence of oral bacteria on titanium surfaces exposed to the oral cavity to maintain plaque-free implants. Previous in vitro studies have shown that titanium implant surfaces coated with titanium nitride (TiN) reduced bacterial colonization compared to other clinically used implant surfaces. The aim of the present study was to examine the support of fibroblast growth by a TiN surface that has antimicrobial characteristics. Mouse fibroblasts were cultured on smooth titanium discs that were either magnetron-sputtered with a thin layer of titanium nitride, thermal oxidized, or modified with laser radiation (using a Nd-YAG laser). The resulting surface topography was examined by scanning electron microscopy (SEM), and surface roughness was estimated using a two-dimensional contact stylus profilometer. A protein assay (BCA assay) and a colorimetric assay to examine fibroblast metabolism (MTT) were used. Cellular morphology and cell spreading were analyzed using SEM and fluorescence microscopy. Fibroblasts on oxidized titanium surfaces showed a more spherical shape, whereas cells on laser-treated titanium and on TiN appeared intimately adherent to the surface. The MTT activity and total protein were significantly increased in fibroblasts cultured on titanium surfaces coated with TiN compared to all other surface modifications tested. This study suggests that a titanium nitride coating might be suitable to support tissue growth on implant surfaces.
设计牙种植体时的一个主要考虑因素是创建一个能在种植体与骨、结缔组织或上皮之间提供牢固附着的表面。此外，抑制口腔细菌在暴露于口腔的钛表面上的黏附，以保持种植体无菌斑，这一点也很重要。先前的体外研究表明，与其他临床使用的种植体表面相比，涂有氮化钛（TiN）的钛种植体表面可减少细菌定植。本研究的目的是检验具有抗菌特性的TiN表面对成纤维细胞生长的支持作用。将小鼠成纤维细胞培养在光滑的钛盘上，这些钛盘要么用一层薄薄的氮化钛进行磁控溅射、热氧化，要么用激光辐射（使用Nd-YAG激光）进行改性。通过扫描电子显微镜（SEM）检查所得的表面形貌，并使用二维接触式触针轮廓仪估计表面粗糙度。采用蛋白质测定法（BCA测定法）和比色法检测成纤维细胞代谢（MTT）。使用SEM和荧光显微镜分析细胞形态和细胞铺展情况。氧化钛表面上的成纤维细胞呈更球形，而激光处理的钛表面和TiN表面上的细胞似乎紧密附着于表面。与所有其他测试的表面改性相比，在涂有TiN的钛表面上培养的成纤维细胞中MTT活性和总蛋白显著增加。本研究表明，氮化钛涂层可能适合支持种植体表面的组织生长。
