Nardone G, Rippa E, Martin G, Rocco A, Siciliano R A, Fiengo A, Cacace G, Malorni A, Budillon G, Arcari P
Department of Clinical and Experimental Medicine, Gastroenterology, Federico II University of Naples, Via S. Pansini, 5 80131 Naples, Italy.
Dig Liver Dis. 2007 Feb;39(2):122-9. doi: 10.1016/j.dld.2006.09.017. Epub 2006 Nov 7.
To understand the molecular changes underlying Helicobacter pylori-related gastric diseases is mandatory to prevent gastric cancer. Proteomic technology is providing a rapid expansion of the basic knowledge, particularly in the discovery of new biomarkers involved in the tumourigenesis.
To characterise changes in protein expression level of the gastric mucosa in H. pylori-infected patients.
The population enrolled comprised 41 dyspeptic patients. Proteins extracted from gastric mucosal specimens were analysed by 2-dimensional electrophoresis, sequenced by MALDI-TOF and identified by Edman's degradation.
Twenty-one out of 41 patients had H. pylori infection of whom 17 had anti-CagA IgG antibodies. Several proteins were identified, of which Rho guanosine diphosphatase dissociation inhibitor alpha and heat shock protein 27 increased and glutathione transferase and antrum mucosa protein-18 decreased in H. pylori-positive in respect to H. pylori-negative patients. Interestingly, antrum mucosa protein-18, currently referred as gastrokine-1, showed two isoforms differing in the first N-terminal amino acid residue. Both gastrokine-1 isoforms were observed in the H. pylori-negative group whereas a lower expression or even absence of the gastrokine-1 basic isoform was found in a subgroup (7/21) of H. pylori-positive patients with moderate-severe gastritis.
Our study demonstrated the presence of gastrokine-1 isoforms of which the basic isoform was reduced in a subset of patients with H. pylori infection.
了解幽门螺杆菌相关胃部疾病背后的分子变化对于预防胃癌至关重要。蛋白质组学技术正在迅速拓展基础知识，尤其是在发现参与肿瘤发生的新生物标志物方面。
对幽门螺杆菌感染患者胃黏膜蛋白质表达水平的变化进行特征描述。
纳入的人群包括41名消化不良患者。从胃黏膜标本中提取的蛋白质通过二维电泳进行分析，通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）进行测序，并通过埃德曼降解法进行鉴定。
41名患者中有21名感染了幽门螺杆菌，其中17名具有抗细胞毒素相关基因A（CagA）免疫球蛋白G（IgG）抗体。鉴定出了几种蛋白质，与幽门螺杆菌阴性患者相比，幽门螺杆菌阳性患者中 Rho 鸟苷二磷酸酶解离抑制剂α和热休克蛋白27增加，而谷胱甘肽转移酶和胃窦黏膜蛋白-18减少。有趣的是，目前被称为胃动素-1的胃窦黏膜蛋白-18显示出两种在第一个N端氨基酸残基上不同的异构体。在幽门螺杆菌阴性组中观察到了两种胃动素-1异构体，而在患有中重度胃炎的幽门螺杆菌阳性患者亚组（7/21）中发现胃动素-1碱性异构体的表达较低甚至缺失。
我们的研究证明了胃动素-1异构体的存在，其中碱性异构体在一部分幽门螺杆菌感染患者中减少。
