Fernie B F, Poli G, Fauci A S
Department of Microbiology, Georgetown University, Rockville, Maryland 20852.
J Virol. 1991 Jul;65(7):3968-71. doi: 10.1128/JVI.65.7.3968-3971.1991.
Alpha interferon (IFN-alpha) is effective in preventing the release of human immunodeficiency virus (HIV) from chronically infected T-lymphocytic (ACH-2) and promonocytic (U1) cell lines stimulated with the phorbol ester phorbol-12-myristate-13 acetate (PMA). In the present study, we observed that together with particle production, shedding of HIV antigen (p24gag) occurs in the T-cell line ACH-2 both constitutively and after stimulation with PMA. IFN-alpha, although effective in suppressing the release of HIV particles, did not inhibit shedding of p24gag into the culture supernatants of either unstimulated or PMA-stimulated cells. These observations may be of relevance in the evaluation of the in vivo efficacy of IFN-alpha treatment of HIV-infected individuals as determined by levels of p24 antigen in plasma.
α干扰素（IFN-α）可有效阻止人免疫缺陷病毒（HIV）从受佛波酯佛波醇-12-肉豆蔻酸酯-13-乙酸酯（PMA）刺激的慢性感染T淋巴细胞系（ACH-2）和原单核细胞系（U1）中释放。在本研究中，我们观察到，在T细胞系ACH-2中，HIV抗原（p24gag）的脱落与病毒颗粒产生同时发生，且在组成性情况下以及PMA刺激后均会出现。IFN-α虽然能有效抑制HIV颗粒的释放，但并未抑制p24gag向未刺激或PMA刺激细胞的培养上清液中的脱落。这些观察结果可能与通过血浆中p24抗原水平评估IFN-α治疗HIV感染个体的体内疗效有关。
