Cotten M, Oberhauser B, Brunar H, Holzner A, Issakides G, Noe C R, Schaffner G, Wagner E, Birnstiel M L
Institute for Molecular Pathology, Vienna, Austria.
Nucleic Acids Res. 1991 May 25;19(10):2629-35. doi: 10.1093/nar/19.10.2629.
We describe the synthesis of 2'-O-methyl, 2'-O-ethyl oligoribonucleotides and phosphorothioate oligodeoxyribonucleotides and demonstrate their utility as inhibitors of the in vitro U7 snRNP-dependent mRNA processing event. These 2'-O-modified compounds were designed to possess the binding affinity of an RNA molecule towards a complementary RNA target with an enhanced stability against nucleases. The 2'-O-methyl and 2'-O-ethyl antisense compounds function as potent inhibitors of the reaction at 1-10 nM, approximately 5-fold more effective than a natural antisense RNA molecule and requiring an approximate 5-fold excess over the target RNA for 80% inhibition of the processing reaction.
