生理和蛋白质谱分析为体外茉莉酸调控马铃薯块茎发育的潜在分子机制提供了深入了解。

Physiological and protein profiling analysis provides insight into the underlying molecular mechanism of potato tuber development regulated by jasmonic acid in vitro.

机构信息

State Key Laboratory of Aridland Crop Science, Gansu Key Laboratory of Crop Improvement & Germplasm Enhancement, College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, China.

College of Horticulture, Gansu Agricultural University, Lanzhou, China.

出版信息

BMC Plant Biol. 2022 Oct 10;22(1):481. doi: 10.1186/s12870-022-03852-x.

DOI:10.1186/s12870-022-03852-x

PMID:36210448

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9549635/

Abstract

BACKGROUND

Jasmonates (JAs) are one of important phytohormones regulating potato tuber development. It is a complex process and the underlying molecular mechanism regulating tuber development by JAs is still limited. This study attempted to illuminate it through the potential proteomic dynamics information about tuber development in vitro regulated by exogenous JA.

RESULTS

A combined analysis of physiological and iTRAQ (isobaric tags for relative and absolute quantification)-based proteomic approach was performed in tuber development in vitro under exogenous JA treatments (0, 0.5, 5 and 50 μΜ). Physiological results indicated that low JA concentration (especially 5 μM) promoted tuber development, whereas higher JA concentration (50 μM) showed inhibition effect. A total of 257 differentially expressed proteins (DEPs) were identified by iTRAQ, which provided a comprehensive overview on the functional protein profile changes of tuber development regulated by JA. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that low JA concentration (especially 5 μM) exhibited the promotion effects on tuber development in various cellular processes. Some cell wall polysaccharide synthesis and cytoskeleton formation-related proteins were up-regulated by JA to promote tuber cell expansion. Some primary carbon metabolism-related enzymes were up-regulated by JA to provide sufficient metabolism intermediates and energy for tuber development. And, a large number of protein biosynthesis, degradation and assembly-related were up-regulated by JA to promote tuber protein biosynthesis and maintain strict protein quality control during tuber development.

CONCLUSIONS

This study is the first to integrate physiological and proteomic data to provide useful information about the JA-signaling response mechanism of potato tuber development in vitro. The results revealed that the levels of a number of proteins involved in various cellular processes were regulated by JA during tuber development. The proposed hypothetical model would explain the interaction of these DEPs that associated with tuber development in vitro regulated by JA.

摘要

背景

茉莉酸（JAs）是调节马铃薯块茎发育的重要植物激素之一。这是一个复杂的过程，JA 调节块茎发育的潜在分子机制仍有限。本研究试图通过外源 JA 处理下体外块茎发育的潜在蛋白质组动态信息来阐明这一过程。

结果

在外源 JA（0、0.5、5 和 50μM）处理下，对体外块茎发育进行了生理和 iTRAQ（相对和绝对定量同位素标记）相结合的蛋白质组分析。生理结果表明，低 JA 浓度（特别是 5μM）促进块茎发育，而较高 JA 浓度（50μM）则表现出抑制作用。通过 iTRAQ 共鉴定到 257 个差异表达蛋白（DEPs），为 JA 调节块茎发育的功能蛋白谱变化提供了全面概述。基因本体论（GO）和京都基因与基因组百科全书（KEGG）通路富集分析表明，低 JA 浓度（特别是 5μM）在各种细胞过程中表现出促进块茎发育的作用。一些细胞壁多糖合成和细胞骨架形成相关蛋白被 JA 上调，以促进块茎细胞扩张。一些初级碳代谢相关酶被 JA 上调，为块茎发育提供充足的代谢中间产物和能量。并且，大量的蛋白质生物合成、降解和组装相关蛋白被 JA 上调，以促进块茎蛋白的生物合成并在块茎发育过程中保持严格的蛋白质质量控制。