Goldie P, Hellström S, Forsgren S
Department of Anatomy, University of Umeå, Sweden.
Arch Otorhinolaryngol. 1989;246(4):183-9. doi: 10.1007/BF00453658.
The distribution and origin of peptide-containing and sympathetic nerve fibers were studied in the tympanic membrane of rats with intact innervation, and in rats following sympathectomy, vagotomy, or capsaicin treatment. Nerve fibers showing substance-P-like immunoreactivity (SP-LI), calcitonin gene-related peptide (CGRP)-LI, vasoactive intestinal polypeptide (VIP)-LI, enkephalin-LI, neuropeptide Y (NPY)-LI and tyrosine hydroxylase (TH)-LI were detected along blood vessels in the pars flaccida, the external auditory canal and in the fibrocartilaginous ring of the pars tensa. In the pars flaccida there were numerous fibers demonstrating SP-LI and CGRP-LI, while there were few such fibers in the pars tensa. In both portions of the tympanic membrane these fibers were present within and beneath the keratinized stratified squamous epithelium. In the pars flaccida, nerve fibers showing SP-LI and CGRP-LI were also seen near mast cells. Sympathectomy led to a loss of nerve fibers showing TH-LI and NPY-LI, whereas the other peptide-containing nerve fibers remained unaffected. Vagotomy did not reduce the immunoreactivity for any of the neuropeptides studied. Capsaicin treatment caused a reduction in nerve fibers displaying SP-LI and CGRP-LI. The abundance of nerve fibers showing SP-LI and CGRP-LI in the keratinized squamous epithelium indicates that the tympanic membrane is richly supplied with sensory nerves. The localization of nerve fibers exhibiting these latter substances in the vicinity of mast cells in the pars flaccida suggests that this part of the tympanic membrane is a site where neurogenic inflammation occurs.
在具有完整神经支配的大鼠鼓膜以及交感神经切除、迷走神经切断或辣椒素处理后的大鼠鼓膜中，研究了含肽神经纤维和交感神经纤维的分布及起源。在松弛部、外耳道以及紧张部的纤维软骨环的血管周围，检测到显示P物质样免疫反应性（SP-LI）、降钙素基因相关肽（CGRP）-LI、血管活性肠多肽（VIP）-LI、脑啡肽-LI、神经肽Y（NPY）-LI和酪氨酸羟化酶（TH）-LI的神经纤维。在松弛部有大量显示SP-LI和CGRP-LI的纤维，而在紧张部此类纤维较少。在鼓膜的这两个部分，这些纤维存在于角化复层鳞状上皮内及其下方。在松弛部，显示SP-LI和CGRP-LI的神经纤维也见于肥大细胞附近。交感神经切除导致显示TH-LI和NPY-LI的神经纤维缺失，而其他含肽神经纤维未受影响。迷走神经切断并未降低所研究的任何神经肽的免疫反应性。辣椒素处理导致显示SP-LI和CGRP-LI的神经纤维减少。角化复层鳞状上皮中显示SP-LI和CGRP-LI的神经纤维丰富，表明鼓膜有丰富的感觉神经供应。松弛部肥大细胞附近显示这些物质的神经纤维定位表明，鼓膜的这一部分是神经源性炎症发生的部位。
