Domeika M A, Bassiri M, Mårdh P A
Institute of Clinical Bacteriology, Uppsala University, Sweden.
Infection. 1994 Mar-Apr;22(2):65-8. doi: 10.1007/BF01739005.
First-void urine specimens from 224 male recruits and 443 patients of venereal disease clinics without complaints of symptoms of urethritis were collected. Urinary leukocyte esterase test, two enzyme immunoassays (EIAs: Syva MicroTrak and Orion), a chemiluminometric assay (Magic Lite) and Syva's MicroTrak direct immunofluorescence test were used. The prevalence of chlamydial urethritis in the study population as determined by direct immunofluorescence test of first-void urine in the military recruits and venereal disease patients was 1.3% and 6.3%, respectively. The denominator used for calculation of sensitivities was the sum of patients with positive test results in at least two of the different test systems used. The sensitivities of first-void urine were 100% for Syva EIA, 96.7% for Orion EIA and 86.7% for the chemiluminometric assay. All assays proved highly specific (99.5-99.7%). Compared with direct immunofluorescence test of first-void urine, the urine leukocyte esterase test had a sensitivity of 93.6% and a specificity of 94.3%. The study showed that the urine leukocyte esterase test is an effective method to detect males infected by Chlamydia trachomatis.
收集了224名男性新兵以及443名性病门诊患者的首次晨尿样本，这些患者均无尿道炎症状主诉。采用了尿白细胞酯酶检测、两种酶免疫测定法（酶免疫测定：Syva MicroTrak和Orion）、化学发光测定法（Magic Lite）以及Syva的MicroTrak直接免疫荧光检测法。通过对新兵和性病患者首次晨尿进行直接免疫荧光检测，研究人群中衣原体尿道炎的患病率分别为1.3%和6.3%。计算敏感度时所用的分母为至少在两种不同检测系统中检测结果呈阳性的患者总数。首次晨尿检测中，Syva酶免疫测定法的敏感度为100%，Orion酶免疫测定法为96.7%，化学发光测定法为86.7%。所有检测方法均具有高度特异性（99.5 - 99.7%）。与首次晨尿直接免疫荧光检测相比，尿白细胞酯酶检测的敏感度为93.6%，特异性为94.3%。该研究表明，尿白细胞酯酶检测是检测男性沙眼衣原体感染的有效方法。
