Owen Chris A, Notingher Ioan, Hill Robert, Stevens Molly, Hench Larry L
Department of Materials, Imperial College London, Prince Consort Road, London, SW7 2AZ, United Kingdom.
J Mater Sci Mater Med. 2006 Nov;17(11):1019-23. doi: 10.1007/s10856-006-0438-6. Epub 2006 Nov 22.
This review summarises progress in Raman spectroscopy and its application in diagnostics, toxicological testing and tissue engineering. Applications of Raman spectroscopy in cell biology are in the early stages of development, however, recent publications have demonstrated its utilisation as a diagnostic and development tool with the key advantage that investigations of living cells can be performed non-invasively.Some of the research highlighted here demonstrates the ability of Raman spectroscopy to accurately characterise cancer cells and distinguish between similar cell types. Many groups have used Raman spectroscopy to study tissues, but recently increased effort has gone into single cell analysis of cell lines; the advantages being that cell lines offer ease of handling and increased reproducibility over tissue studies and primary cells. The main goals of bio-Raman spectroscopy at this stage are twofold. Firstly, the aim is to further develop the diagnostic ability of Raman spectroscopy so it can be implemented in a clinical environment, producing accurate and rapid diagnoses. Secondly, the aim is to optimise the technique as a research tool for the non-invasive real time investigation of cell/material interactions in the fields of tissue engineering and toxicology testing.
本综述总结了拉曼光谱学的进展及其在诊断、毒理学测试和组织工程中的应用。拉曼光谱学在细胞生物学中的应用尚处于发展初期，然而，最近的出版物已证明其作为一种诊断和开发工具的用途，其关键优势在于可以对活细胞进行非侵入性研究。这里重点介绍的一些研究展示了拉曼光谱学准确表征癌细胞并区分相似细胞类型的能力。许多研究小组已使用拉曼光谱学研究组织，但最近对细胞系的单细胞分析投入了更多精力；优势在于与组织研究和原代细胞相比，细胞系更易于操作且重复性更高。现阶段生物拉曼光谱学的主要目标有两个。其一，旨在进一步提高拉曼光谱学的诊断能力，以便能够在临床环境中应用，实现准确、快速的诊断。其二，旨在优化该技术，使其成为组织工程和毒理学测试领域中用于细胞/材料相互作用非侵入性实时研究的研究工具。
