Gambi D, Porrini A M, Giampietro A, Macor S
Department of Clinical Neurology, University of Chieti, Italy.
J Neuroimmunol. 1991 Aug;33(2):97-102. doi: 10.1016/0165-5728(91)90053-a.
Two-color flow cytometric analysis on peripheral blood lymphocytes of 35 untreated multiple sclerosis (MS) patients, 17 other medical disease (OMD) patients and 14 healthy control (HC) subjects was performed to evaluate the levels of different T and B cell subpopulations. In MS patients we observed an increase in CD4+CD29+ helper-inducer cells but this increase was not related to the different phases of the disease. We hypothesize that this change is related to the reduction of CD21+ cells expressing B2 antigen, a 140 kDa molecule disappearing after B cell activation. An increased level of CD4+CD45RA- (helper-inducer-like cells) and a reduction of CD4+CD29- (suppressor-inducer-like cells) were also present in our patients. These findings demonstrate an immune 'disequilibrium' in MS, which is linked with an increased level of CD25+ cells expressing the interleukin-2 (IL-2) receptor. IL-2, besides being a T cell growth factor, is also a B cell growth factor. These data let us hypothesize that an activation of the immune response is present in MS.
对35例未经治疗的多发性硬化症（MS）患者、17例其他内科疾病（OMD）患者和14例健康对照（HC）受试者的外周血淋巴细胞进行双色流式细胞术分析，以评估不同T和B细胞亚群的水平。在MS患者中，我们观察到CD4+CD29+辅助诱导细胞增加，但这种增加与疾病的不同阶段无关。我们推测这种变化与表达B2抗原的CD21+细胞减少有关，B2抗原是一种140 kDa的分子，在B细胞激活后消失。我们的患者中还存在CD4+CD45RA-（辅助诱导样细胞）水平升高和CD4+CD29-（抑制诱导样细胞）减少。这些发现表明MS存在免疫“失衡”，这与表达白细胞介素-2（IL-2）受体的CD25+细胞水平升高有关。IL-2除了是一种T细胞生长因子外，也是一种B细胞生长因子。这些数据使我们推测MS中存在免疫反应激活。
