糖基化抑制因子介导子宫内膜中血管内皮生长因子因氧化应激而增加。

Glycodelin mediates the increase in vascular endothelial growth factor in response to oxidative stress in the endometrium.

作者信息

Park John K, Song MingQing, Dominguez Celia E, Walter Mary F, Santanam Nalini, Parthasarathy Sampath, Murphy Ana A

机构信息

Department of Gynecology and Obstetrics, Emory University School of Medicine, Atlanta, GA, USA.

出版信息

Am J Obstet Gynecol. 2006 Dec;195(6):1772-7. doi: 10.1016/j.ajog.2006.07.025.

DOI:10.1016/j.ajog.2006.07.025

PMID:17132480

Abstract

OBJECTIVE

We hypothesized that glycodelin stimulates vascular endothelial growth factor (VEGF) expression in response to oxidative stress.

STUDY DESIGN

EM42 (human endometrial epithelial cell line) and primary endometrial epithelial cells were subjected to oxidative stress with minimally oxidized low density lipoprotein (mLDL). Cells were also incubated with no LDL (control) or native LDL (nLDL). Each condition was incubated with and without glycodelin antibody. Glycodelin and VEGF protein and messenger RNA (mRNA) levels were analyzed. Primary cells were cultured with glycodelin peptide to evaluate the effect on VEGF protein and mRNA.

RESULTS

Glycodelin and VEGF protein and mRNA were higher for cells grown with mLDL (P < .05), while glycodelin antibody attenuated the increase in VEGF protein (P < .01). Glycodelin peptide increased VEGF mRNA and protein (P < .05).

CONCLUSION

Glycodelin may act as an autocrine factor within endometriotic implants to increase VEGF expression during oxidative stress.

摘要

目的

我们推测，在氧化应激反应中，糖蛋白会刺激血管内皮生长因子（VEGF）的表达。

研究设计

用轻度氧化的低密度脂蛋白（mLDL）使EM42（人子宫内膜上皮细胞系）和原代子宫内膜上皮细胞遭受氧化应激。细胞也分别与无低密度脂蛋白（对照）或天然低密度脂蛋白（nLDL）一起孵育。每种条件下的细胞都分别在有和无糖蛋白抗体的情况下进行孵育。分析了糖蛋白、VEGF的蛋白质和信使核糖核酸（mRNA）水平。用糖蛋白肽培养原代细胞，以评估其对VEGF蛋白质和mRNA的影响。