Kuang P, Wu W, Liu J, Zhang F, Pu C
Neurotransmitter Research Laboratory, Chinese PLA General Hospital, Postgraduate Military Madical School, Beijing.
J Tradit Chin Med. 1991 Jun;11(2):123-7.
The levels of substance P (SP) in rat brains were assayed in 64 rats. Bilateral common carotid artery ligation was done in 49 rats. Half an hour before ligation, 25 rats were given 10 g/kg of RSM; 24 rats were given the same volume of normal saline as controls. Sham operation was done in 15 rats. Half an hour and 3 hours after cerebral ischemia, the rats were quickly decapitated. SP concentration was assayed in the cerebral cortex, caudate nucleus and brain stem. In saline-treated animals, the SP level of caudate nucleus at 3-hour group was significantly decreased as compared with the 0.5-hour group and sham-operated group respectively. No significances were found among RSM-treated groups and sham-operated groups. The SP levels were shown: brain stem greater than caudate nucleus greater than cerebral cortex. The preliminary results suggest that SP may be involved in the pathophysiologic procedures of cerebral ischemia and RSM may attenuate the dysfunction of SP during cerebral ischemia.
对64只大鼠的大鼠脑内P物质（SP）水平进行了测定。49只大鼠进行双侧颈总动脉结扎。结扎前半小时，25只大鼠给予10 g/kg的丹参；24只大鼠给予相同体积的生理盐水作为对照。15只大鼠进行假手术。脑缺血后半小时和3小时，迅速将大鼠断头。测定大脑皮层、尾状核和脑干中的SP浓度。在生理盐水处理的动物中，3小时组尾状核的SP水平分别与0.5小时组和假手术组相比显著降低。在丹参处理组和假手术组之间未发现显著差异。SP水平显示为：脑干＞尾状核＞大脑皮层。初步结果表明，SP可能参与脑缺血的病理生理过程，丹参可能减轻脑缺血期间SP的功能障碍。
