Nakamura Y, Kawazu S, Minami T
First Research Division, National Institute of Animal Health, Ibaraki, Japan.
J Vet Med Sci. 1991 Feb;53(1):73-9. doi: 10.1292/jvms.53.73.
Protein composition was compared and epitopes were analyzed among the isolates of Anaplasma centrale and A. marginale by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting using bovine antisera and monoclonal antibodies, and enzyme-linked immunosorbent assay. Common and unique proteins were found among the isolates. All isolates tested had a major surface protein with an apparent molecular weight of 38 to 40 kilodalton which had slight molecular size variations between species. This protein was also a dominant immunogen to the host. At least two species-common epitopes, one of which might contain carbohydrate(s), were present on the major surface protein. One species-specific epitope was identified on the major surface protein of A. marginale isolates.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、使用牛抗血清和单克隆抗体的免疫印迹以及酶联免疫吸附测定，对中央无形体和边缘无形体的分离株进行了蛋白质组成比较并分析了表位。在分离株中发现了共同和独特的蛋白质。所有测试的分离株都有一种主要表面蛋白，其表观分子量为38至40千道尔顿，不同物种之间的分子大小略有差异。这种蛋白质也是宿主的主要免疫原。主要表面蛋白上存在至少两个物种共有的表位，其中一个可能含有碳水化合物。在边缘无形体分离株的主要表面蛋白上鉴定出一个物种特异性表位。
