Abramovic Z, Sentjurc M, Kristl J, Khan N, Hou H, Swartz H M
Laboratory for Biophysics, Jozef Stefan Institute, Ljubljana, Slovenia.
Skin Pharmacol Physiol. 2007;20(2):77-84. doi: 10.1159/000097654. Epub 2006 Dec 1.
The effects of two general anesthetics on skin oxygenation in mice are evaluated by electron paramagnetic resonance oximetry. Up to now no data on the effects of different anesthetics on skin oxygenation could be found. In this study animals were anesthetized with ketamine/xylazine or isoflurane, and partial pressure of oxygen (pO(2)) in the skin, heart rate and hemoglobin oxygen saturation were followed as a function of time and inhaled oxygen concentration. The skin pO(2) significantly increased continuously for about 60 min in mice anesthetized with isoflurane and remained constant after that. During ketamine/xylazine anesthesia, the pO(2) in the skin only slightly decreased. The skin pO(2) increased with higher inspired oxygen concentrations for both anesthetics groups. When breathing 21% oxygen, mice anesthetized with isoflurane had two-fold higher pO(2) in the skin compared to mice anesthetized with ketamine/xylazine. The heart rate was significantly lower in animals anesthetized with ketamine/xylazine, while hemoglobin saturation was almost the same in both groups at all inhaled oxygen concentrations. These results show that the type of anesthesia is an important parameter that needs to be considered in experiments where skin pO(2) is followed.
通过电子顺磁共振血氧测定法评估两种全身麻醉剂对小鼠皮肤氧合的影响。到目前为止，尚未发现关于不同麻醉剂对皮肤氧合影响的数据。在本研究中，动物用氯胺酮/赛拉嗪或异氟烷麻醉，并跟踪皮肤中的氧分压（pO₂）、心率和血红蛋白氧饱和度随时间和吸入氧浓度的变化。在用异氟烷麻醉的小鼠中，皮肤pO₂在约60分钟内持续显著增加，之后保持恒定。在氯胺酮/赛拉嗪麻醉期间，皮肤中的pO₂仅略有下降。两个麻醉组的皮肤pO₂均随吸入氧浓度升高而增加。当呼吸21%的氧气时，用异氟烷麻醉的小鼠皮肤中的pO₂是用氯胺酮/赛拉嗪麻醉的小鼠的两倍。用氯胺酮/赛拉嗪麻醉的动物心率显著较低，而在所有吸入氧浓度下，两组的血红蛋白饱和度几乎相同。这些结果表明，在跟踪皮肤pO₂的实验中，麻醉类型是一个需要考虑的重要参数。
