Goretsky M J, Supp A P, Greenhalgh D G, Warden G D, Boyce S T
Department of Surgery, University of Cincinnati, Cincinnati, Ohio 45229, USA.
Wound Repair Regen. 1995 Oct-Dec;3(4):419-25. doi: 10.1046/j.1524-475X.1995.30406.x.
Restoration of the epidermal barrier is a requirement for burn wound closure. A rapid, reliable, and noninvasive measure of the rate of restoration of the epidermal barrier is not readily available. To monitor the reformation of the epidermal barrier, we measured surface electrical capacitance on cultured skin substitutes (human keratinocytes and fibroblasts attached to collagen-glycosaminoglycan substrates) and split-thickness skin autografts grafted to patients. Data were collected from four patients with burns and one pediatric patient with a congenital hairy nevus comprising > 60% total body surface area. Capacitance measurements were performed at days 7, 10, 12, 14, and 28 by direct contact of the capacitance probe for 10 seconds to the cultured skin substitutes or split-thickness autograft. On postoperative days 7, 10, 12, 14, 21, and 28, the surface electrical capacitance of cultured skin substitutes after 10 seconds of sampling was 2468 +/- 268, 1443 +/- 439, 129 +/- 43, 200 +/- 44, 88 +/- 20, and 74 +/- 19 picofarads (mean +/- standard error of the mean), respectively. Surface electrical capacitance for split-thickness autograft on the same days was 1699 +/- 371, 1914 +/- 433, 125 +/- 16, 175 +/- 63, 110 +/- 26, 271 +/- 77 picofarads, respectively. Surface electrical capacitance in all of the grafts decreased with time. Cultured skin substitutes had approximately the same 10-second capacitance values as split-thickness autograft during 3 weeks of healing and approached values for uninjured skin (32 +/- 5 picofarads) by 12 days. Measurement of surface electrical capacitance is a direct, inexpensive, and convenient index for noninvasive monitoring of epidermal barrier formation.
表皮屏障的恢复是烧伤创面愈合的必要条件。目前尚缺乏一种快速、可靠且无创的方法来测量表皮屏障的恢复速率。为了监测表皮屏障的重建过程，我们对培养的皮肤替代物（附着于胶原 - 糖胺聚糖基质上的人角质形成细胞和成纤维细胞）以及移植到患者身上的断层自体皮片进行了表面电容测量。数据收集自4名烧伤患者和1名先天性多毛痣累及全身表面积>60%的儿科患者。在第7、10、12、14和28天，通过将电容探头直接接触培养的皮肤替代物或断层自体皮片10秒钟来进行电容测量。术后第7、10、12、14、21和28天，采样10秒后培养的皮肤替代物的表面电容分别为2468±268、1443±439、129±43、200±44、88±20和74±19皮法（平均值±平均标准误差）。同一时期断层自体皮片的表面电容分别为1699±371、1914±433、125±16、175±63、110±26、271±77皮法。所有移植皮片的表面电容均随时间下降。在愈合的3周内，培养的皮肤替代物的10秒电容值与断层自体皮片大致相同，并在12天时接近未受伤皮肤的电容值（32±5皮法）。表面电容测量是一种直接、廉价且方便的无创监测表皮屏障形成的指标。
