Aboufaycal H, Sader H S, Rolston K, Deshpande L M, Toleman M, Bodey G, Raad I, Jones R N
The University of Texas M.D. Anderson Cancer Center, Houston, TX 77030, USA.
J Clin Microbiol. 2007 Feb;45(2):614-5. doi: 10.1128/JCM.01351-06. Epub 2006 Dec 6.
Two Pseudomonas aeruginosa strains resistant to beta-lactams, fluoroquinolones, aminoglycosides, tetracyclines, and carbapenems and susceptible only to polymyxin B (MIC <or= 2 microg/ml) were identified as part of the Meropenem Yearly Susceptibility Test Information Collection program. Metallo-beta-lactamase screening tests were positive, PCR yielded products with blaVIM primers, and sequence analysis revealed blaVIM-7 and blaVIM-2. The isolates had distinct ribotype and pulsed-field gel electorphoresis patterns and appeared independently, remote in time and location, at the same cancer center.
作为美罗培南年度药敏试验信息收集项目的一部分,鉴定出两株对β-内酰胺类、氟喹诺酮类、氨基糖苷类、四环素类和碳青霉烯类耐药且仅对多粘菌素B敏感(MIC≤2μg/ml)的铜绿假单胞菌菌株。金属β-内酰胺酶筛选试验呈阳性,PCR扩增出blaVIM引物的产物,序列分析显示为blaVIM-7和blaVIM-2。这些分离株具有不同的核糖体分型和脉冲场凝胶电泳图谱,且在同一癌症中心独立出现,时间和地点相隔较远。