Chou Szu-Yuan, Hsu Chun-Sen, Wang Kun-Teng, Wang Min-Chieh, Wang Ching-Chiung
Department of Obstetrics and Gynecology, Taipei Medical University-Wan Fang Medical Center, Taipei, Taiwan.
Phytother Res. 2007 Mar;21(3):226-30. doi: 10.1002/ptr.2044.
Cnidium monnieri (L.) Cusson is a Chinese medicine which is used widely by traditional medicine doctors. Osthol is a major bio-activity compound of the herb. In this study, osthol was isolated from C. monnieri and its in vitro and in vivo antitumor effects studied. The results of the in vitro study showed: that osthol inhibited the growth of HeLa, in a time- and concentration-dependent manner, with IC(50) values of 77.96 and 64.94 microm for 24 and 48 h, respectively; that osthol had lower cytotoxic effects in primary cultured normal cervical fibroblasts; and that increased DNA fragmentation and activated PARP in HeLa after treatment with osthol which could induce apoptosis. The results of the in vivo model showed that the survival days of the P-388 D1 tumor-bearing CDF(1) mice were prolonged (ILS% = 37) after osthol (30 mg/kg) was given once a day for 9 days. Based on these results, it is suggested that osthol could inhibit P-388 D1 cells in vivo and induce apoptosis in HeLa cells in vitro, and that osthol is good lead compound for developing antitumor drugs. However, C. formosanum Yabe of Taiwan's endemic plants contained little osthol, with no imperatorin, and its major components were different from that of C. monnieri. Therefore, it is suggested that C. formosanum also may possess economic worth.
蛇床子是一种被传统医学医生广泛使用的中药。蛇床子素是该草药的主要生物活性化合物。在本研究中，从蛇床子中分离出蛇床子素，并对其体外和体内抗肿瘤作用进行了研究。体外研究结果表明：蛇床子素以时间和浓度依赖性方式抑制HeLa细胞生长，24小时和48小时的IC(50)值分别为77.96和64.94微摩尔；蛇床子素对原代培养的正常宫颈成纤维细胞的细胞毒性较低；用蛇床子素处理HeLa细胞后，DNA片段化增加且PARP激活，可诱导细胞凋亡。体内模型结果显示，给携带P-388 D1肿瘤的CDF(1)小鼠每天一次给予蛇床子素（30毫克/千克），连续9天，其存活天数延长（ILS% = 37）。基于这些结果，表明蛇床子素在体内可抑制P-388 D1细胞，在体外可诱导HeLa细胞凋亡，且蛇床子素是开发抗肿瘤药物的良好先导化合物。然而，台湾本土植物台湾蛇床几乎不含蛇床子素，没有欧前胡素，其主要成分与蛇床子不同。因此，表明台湾蛇床也可能具有经济价值。
