Cianciolo G J, Jensen J A
Transplantation. 1977 Apr;23(4):303-9. doi: 10.1097/00007890-197704000-00002.
Pooled serum from 6- to 8-week-old female Lewis x Brown Norwegian F1 hybrid (LBNF1) rats undergoing acute local graft-versus-host (GVH) reactions was found to have the ability, when compared with normal F1 serum, to increase node weight in a popliteal lymph node weight gain assay in syngeneic animals. Suspension of donor cells in normal F1 serum on one side and corresponding concentrations of GVH serum on the contralateral side resulted in an average increase in popliteal node weight of 28.8 per cent on the side receiving GVH serum. Of the 64 animals tested, 50 had larger nodes on the GVH serum side. This effect was not impaired by heating (56 C, 30 min) or by rapid freezing (-75 C) and thawing of the serum. Preinjection of the serum into the footpads 2 hr before donor cells also gave increased node weights as did injection of the serum i.p. In contrast, GVH serum was found to inhibit unidirectional mixed lymphocyte cultures of seven different allogeneic rat strain combinations, the inhibition ranging from 35 to 74 per cent. In addition, the blastogenic cell responses of three different strains to phytohemagglutinin P were inhibited between 45 and 77 per cent. The relationship between these two phenomena, in vivo increase in node weight and in vitro inhibition of blastogenesis, is not clear and is the object of further investigation.
研究发现，与正常F1血清相比，6至8周龄雌性Lewis×Brown挪威F1杂交（LBNF1）大鼠在发生急性局部移植物抗宿主（GVH）反应时的混合血清，在同基因动物的腘窝淋巴结增重试验中具有增加淋巴结重量的能力。一侧将供体细胞悬浮于正常F1血清中，另一侧使用相应浓度的GVH血清，结果显示，接受GVH血清一侧的腘窝淋巴结重量平均增加了28.8%。在接受测试的64只动物中，有50只动物的GVH血清侧淋巴结更大。加热（56℃，30分钟）或血清快速冷冻（-75℃）及解冻均未削弱这种效应。在注入供体细胞前2小时将血清预先注入脚垫，以及腹腔注射血清，均能使淋巴结重量增加。相比之下，发现GVH血清可抑制七种不同异基因大鼠品系组合的单向混合淋巴细胞培养，抑制率在35%至74%之间。此外，三种不同品系对植物血凝素P的致有丝分裂细胞反应受到45%至77%的抑制。淋巴结重量在体内增加和体外对细胞增殖的抑制这两种现象之间的关系尚不清楚，有待进一步研究。
