Subba Rao D S, Grogan J B
Transplantation. 1979 Feb;27(2):75-8. doi: 10.1097/00007890-197902000-00001.
The graft-versus-host (GVH) response of spleen cells from rats bearing either orthotopic skin grafts or allogeneic implants in the anterior chamber of the eye was evaluated using popliteal lymph node (PLN) assay. When a viable implant remained in the anterior chamber, the spleen cells of these rats produced a popliteal lymph node enlargement in F1 hybrids which was approximately 50% of that produced by a similar number of cells from a normal animal. Conversely, the GVH response of spleen cells from orthotopically skin-grafted rats was noted to be significantly increased over the response of spleen cells from normal animals. The decrease in the GVH response of implanted rat spleen cells was a specific reaction and not because of trauma or implantation, since spleen cells from rats bearing syngeneic implants had shown no reduction in their GVH-inducing ability. The PLN weights of rats receiving mixed population of normal and implanted rat spleen cells were always less than the weights observed with an equal number of normal spleen cells. These findings permit the assumption that implant-bearing rats may be lacking or low in cells that induce GVH reactions or that there is a delayed conversion of effector cells after early immune recognition.
采用腘窝淋巴结（PLN）测定法评估了原位皮肤移植或眼前房同种异体植入大鼠的脾细胞的移植物抗宿主（GVH）反应。当有活力的植入物留在前房时，这些大鼠的脾细胞在F1杂种中引起腘窝淋巴结肿大，其肿大程度约为正常动物相同数量细胞所引起肿大程度的50%。相反，原位皮肤移植大鼠的脾细胞的GVH反应比正常动物脾细胞的反应显著增强。植入大鼠脾细胞的GVH反应降低是一种特异性反应，并非由于创伤或植入，因为携带同基因植入物大鼠的脾细胞在GVH诱导能力方面未显示降低。接受正常大鼠和植入大鼠脾细胞混合群体的大鼠的PLN重量总是低于等量正常脾细胞所观察到的重量。这些发现使我们可以假设，携带植入物的大鼠可能缺乏诱导GVH反应的细胞或此类细胞数量较少，或者在早期免疫识别后效应细胞存在延迟转化。
