Matković Kristina, Vucemilo Marija, Vinković Bara, Seol Branka, Pavicić Zeljko, Tofant Alenka, Matković Srećko
Department of Animal Hygiene, Environment and Ethology, School of Veterinary Medicine, University of Zagreb, Heinzelova 55, HR-10000 Zagreb, Croatia.
Ann Agric Environ Med. 2006;13(2):349-54.
The main microclimate parameters, i.e. bacterial count and airborne emission to the immediate environment, were analyzed in a dairy barn. Air temperature, relative humidity and air flow velocity were measured on an attested Testo 400 device (Testo Inc., Germany). Air samples were collected by use of a Merck MAS-100 device (Merck KgaA, Darmstadt, Germany) onto a commercially available nutrient Columbia agar (Biolife, Milan, Italy) and incubated for 24 h in an incubator at 37 degrees C work temperature. Measurements were carried out once a week in the morning, at noon, and in the evening during October and November 2002. In the barn, measurements were performed in the animal housing area along the feedlot, and outside the barn at a distance of 5 m, 25 m and 50 m eastward and westward from the barn. The measured dairy barn temperature ranged from 11.2 degrees C to 13.1 degrees C, relative humidity from 71.3-78.6 %, and air flow velocity from 0.09-0.11 m/s. The mean value of total bacterial count in the barn air ranged from 2.82 x 10 (4) cfu/m(3) at noon to 7.76 x 10(4) cfu/m(3) in the evening. Bacterial count decreased at particular measuring sites outside the barn, with Wilcoxon matched pair test showing statistical significance (p < 0.05) at a distance of 5 m eastward and 5 m westward of the barn.
在一个奶牛场中分析了主要的小气候参数，即细菌数量和向周围环境的空气排放。使用经过认证的德图400设备（德国德图公司）测量气温、相对湿度和气流速度。使用默克MAS - 100设备（德国达姆施塔特默克股份两合公司）将空气样本采集到市售的营养哥伦比亚琼脂（意大利米兰生物生命公司）上，并在37摄氏度工作温度的培养箱中培养24小时。在2002年10月和11月期间，每周在上午、中午和晚上进行一次测量。在牛舍内，沿着饲养场在动物饲养区进行测量，在牛舍外，从牛舍向东和向西5米、25米和50米处进行测量。测得的奶牛场温度范围为11.2摄氏度至13.1摄氏度，相对湿度为71.3 - 78.6%，气流速度为0.09 - 0.11米/秒。牛舍空气中细菌总数的平均值范围从中午的2.82×10⁴cfu/m³到晚上的7.76×10⁴cfu/m³。在牛舍外特定测量点细菌数量减少，威尔科克森配对检验显示在牛舍向东和向西5米处具有统计学显著性（p < 0.05）。
