Wright-Williams Siân L, Courade Jean-Philippe, Richardson Claire A, Roughan John V, Flecknell Paul A
Comparative Biology Centre, University of Newcastle upon Tyne, Framlington Place, Newcastle upon Tyne NE2 4HH, UK.
Pain. 2007 Jul;130(1-2):108-18. doi: 10.1016/j.pain.2006.11.003. Epub 2006 Dec 28.
Behaviour was assessed in 32 C57BL/6JCrl and 32 C3H/HeN male mice 1 h following vasectomy; saline or meloxicam was administered 30 min prior to surgery at 5, 10, or 20 mg kg(-1). Faeces were collected 24 h prior to, and 3, 6, 9, 12, 24 h following, vasectomy for measurement of faecal corticosterone. Peak corticosterone levels were significantly higher in mice that underwent vasectomy and received saline (p<0.001) or meloxicam at 5 or 10 mg kg(-1) (p=0.021, and p<0.001, respectively) compared with normal un-operated controls. Mice that underwent vasectomy and received 20 mg kg(-1) meloxicam had peak corticosterone levels that were not different from normal un-operated mice (p=0.254). Discriminant analysis was used to identify behaviours responsible for group separation; these were summed to create two behaviour scores. Score 2 (the frequency of flinching, writhing, rear leg lift and press 2) was thought to be pain related; mice that underwent vasectomy and received saline exhibited significantly more of these behaviours than the normal controls (p=0.032), and the mice that received meloxicam (at any dose). Strain differences were observed in both the stress response to vasectomy and the behavioural changes; the C3H/HeN mice had higher pain scores (behaviour Score 2) and peak corticosterone responses than the C57BL/6JCrl mice. We have demonstrated that significant changes occur in the behaviour of mice following vasectomy, and these changes are reduced by use of meloxicam. Vasectomy elicits a rise in corticosterone levels that was only reduced by the highest dose of meloxicam.
在输精管切除术后1小时，对32只C57BL/6JCrl雄性小鼠和32只C3H/HeN雄性小鼠的行为进行评估；在手术前30分钟，以5、10或20mg/kg(-1)的剂量给予生理盐水或美洛昔康。在输精管切除术前24小时以及术后3、6、9、12、24小时收集粪便，用于测量粪便皮质酮。与未手术的正常对照组相比，接受生理盐水或5mg/kg(-1)或10mg/kg(-1)美洛昔康的输精管切除小鼠的皮质酮峰值水平显著更高（p<0.001、p=0.021和p<0.001）。接受20mg/kg(-1)美洛昔康的输精管切除小鼠的皮质酮峰值水平与未手术的正常小鼠无差异（p=0.254）。采用判别分析来确定导致组间分离的行为；将这些行为汇总以创建两个行为评分。评分2（畏缩、扭动、后腿抬起和按压2的频率）被认为与疼痛相关；接受生理盐水的输精管切除小鼠比正常对照组以及接受美洛昔康（任何剂量）的小鼠表现出更多的这些行为（p=0.032）。在对输精管切除术的应激反应和行为变化方面均观察到品系差异；C3H/HeN小鼠的疼痛评分（行为评分2）和皮质酮峰值反应高于C57BL/6JCrl小鼠。我们已经证明输精管切除术后小鼠的行为会发生显著变化，而使用美洛昔康可减少这些变化。输精管切除术会引起皮质酮水平升高，只有最高剂量的美洛昔康才能降低这种升高。
