Tubbs Jacquelyn T, Kissling Grace E, Travlos Greg S, Goulding David R, Clark James A, King-Herbert Angela P, Blankenship-Paris Terry L
Cellular and Molecular Pathology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA.
J Am Assoc Lab Anim Sci. 2011 Mar;50(2):185-91.
C57BL/6NCrl male mice (n = 60; age, 6 to 7 wk) underwent partial hepatectomy or no surgery and were given 1 of 3 analgesics pre- and postoperatively. Food and water consumption, body weight, running wheel activity, locomotor activity, and serum corticosterone concentrations were measured before and after surgery. Mice that were surgically manipulated weighed significantly less on days 1 through 3 after surgery than did mice not manipulated surgically. On the day of surgery, the surgery groups consumed significantly less feed (-1.5±0.35 g) than did nonsurgery groups. There were no differences in water consumption on any day between surgery and nonsurgery groups or among the 3 analgesic groups. For running wheel activity, significant decreases in the surgery groups were seen at day 1 after surgery compared with baseline. Surgery groups that received buprenorphine and meloxicam returned to baseline activity levels on day 2 after surgery. Open-field testing revealed no significant differences in locomotor activity in any groups; however, posttreatment locomotor activity in the buprenorphine nonsurgery group was increased compared with baseline, and posttreatment locomotor activity in the flunixin meglumine surgery group was decreased compared with baseline. Serum corticosterone concentrations were within normal limits regardless of treatment in all groups. Comparison of the overall results indicated that meloxicam and buprenorphine, at the dose given, appear to be suitable postoperative analgesics for partial hepatectomy in mice. Flunixin meglumine at the given dosage (2.5 mg/kg) may not provide adequate analgesia for partial hepatectomy.
C57BL/6NCrl雄性小鼠（n = 60；年龄6至7周）接受部分肝切除术或未接受手术，并在术前和术后给予3种镇痛药中的1种。在手术前后测量食物和水的消耗量、体重、跑轮活动、自发活动以及血清皮质酮浓度。接受手术操作的小鼠在术后第1至3天的体重明显低于未接受手术操作的小鼠。在手术当天，手术组消耗的饲料（-1.5±0.35 g）明显少于非手术组。手术组和非手术组之间以及3个镇痛组之间在任何一天的水消耗量均无差异。对于跑轮活动，与基线相比，手术组在术后第1天明显减少。接受丁丙诺啡和美洛昔康的手术组在术后第2天恢复到基线活动水平。旷场试验显示任何组的自发活动均无显著差异；然而，丁丙诺啡非手术组治疗后的自发活动较基线增加，氟尼辛葡甲胺手术组治疗后的自发活动较基线减少。所有组无论接受何种治疗，血清皮质酮浓度均在正常范围内。总体结果比较表明，给予的剂量下，美洛昔康和丁丙诺啡似乎是小鼠部分肝切除术后合适的镇痛药。给予剂量（2.5 mg/kg）的氟尼辛葡甲胺可能无法为部分肝切除术提供足够的镇痛效果。
