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Structural basis of glmS ribozyme activation by glucosamine-6-phosphate.6-磷酸葡萄糖胺激活glmS核酶的结构基础
Science. 2006 Sep 22;313(5794):1752-6. doi: 10.1126/science.1129666.
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Crystal structures of the thi-box riboswitch bound to thiamine pyrophosphate analogs reveal adaptive RNA-small molecule recognition.与硫胺素焦磷酸类似物结合的硫盒核糖开关的晶体结构揭示了适应性RNA-小分子识别。
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Chem Biol. 2006 Aug;13(8):857-68. doi: 10.1016/j.chembiol.2006.06.010.
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Structure of the S-adenosylmethionine riboswitch regulatory mRNA element.S-腺苷甲硫氨酸核糖开关调控mRNA元件的结构
Nature. 2006 Jun 29;441(7097):1172-5. doi: 10.1038/nature04819.
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Structural basis for gene regulation by a thiamine pyrophosphate-sensing riboswitch.硫胺素焦磷酸感应核糖开关对基因调控的结构基础
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Structure of the eukaryotic thiamine pyrophosphate riboswitch with its regulatory ligand.具有调节性配体的真核硫胺素焦磷酸核糖开关的结构
Science. 2006 May 26;312(5777):1208-11. doi: 10.1126/science.1128451. Epub 2006 May 4.
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Thermodynamic and kinetic characterization of ligand binding to the purine riboswitch aptamer domain.配体与嘌呤核糖开关适体结构域结合的热力学和动力学表征。
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8
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10
Riboswitches and the role of noncoding RNAs in bacterial metabolic control.核糖开关及非编码RNA在细菌代谢控制中的作用。
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腺嘌呤核糖开关适体与配体结合的核心要求。

Core requirements of the adenine riboswitch aptamer for ligand binding.

作者信息

Lemay Jean-François, Lafontaine Daniel A

机构信息

Département de biologie, Faculté des sciences, Université de Sherbrooke, Québec, Canada.

出版信息

RNA. 2007 Mar;13(3):339-50. doi: 10.1261/rna.142007. Epub 2007 Jan 2.

DOI:10.1261/rna.142007
PMID:17200422
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1800517/
Abstract

The adenine riboswitch aptamer, the A box, positively regulates gene expression upon adenine binding. To provide insight into structure-function relationships, important for the adenine riboswitch aptamer, we have created alignments for six aptamer sequences that reveal the core requirements. In addition, 2-aminopurine (2AP) binding studies have been used to test the consensus sequence derived from the alignment. Overall, the consensus secondary structure is consistent with 2AP binding studies. However, a position in the core, previously identified as variable, shows restriction in nucleotide sequence. Furthermore, this restriction is found to be related with the ligand specificity of the riboswitch. The implications of this relationship for the riboswitch gene regulation mechanism are discussed.

摘要

腺嘌呤核糖开关适体（A盒）在结合腺嘌呤后正向调节基因表达。为了深入了解对腺嘌呤核糖开关适体至关重要的结构-功能关系，我们对六个适体序列进行了比对，揭示了核心要求。此外，已利用2-氨基嘌呤（2AP）结合研究来测试从比对中得出的共有序列。总体而言，共有二级结构与2AP结合研究结果一致。然而，核心区域中一个先前被确定为可变的位置显示出核苷酸序列的限制。此外，发现这种限制与核糖开关的配体特异性有关。讨论了这种关系对核糖开关基因调控机制的影响。