Yang Ji-Yeon, Lee Sang-Jun, Park Hyun-Woo, Cha Youn-Soo
Research Center of Bioactive Materials, Chonbuk National University, Jeonju, Jeonbuk, Republic of Korea.
J Med Food. 2006 Winter;9(4):459-67. doi: 10.1089/jmf.2006.9.459.
Soy products are mainly composed of proteins, phytochemicals such as isoflavones, soy lipids, and carbohydrates. It is unclear whether an individual component alone or a combined effect of multiple bioactive compounds contributes to the beneficial properties of soy. We investigated the effect of dietary genistein (the principal soy isoflavone) alone and combined with L-carnitine to evaluate possible synergistic effects on the intentionally induced prediabetic state characterized by insulin resistance and obesity in C57Bl/6J mice fed a high-fat diet (HD). In the HD-alone group, abdominal and back fat relative to total body weight were significantly higher compared with other groups including those fed normal diet (ND). Among the HD groups, final weight gains of the HD plus genistein (HD+G) and HD plus genistein plus L-carnitine (HD+G+C) groups were lower compared with that of the control (HD-alone). Especially in liver, the results showed that genistein with carnitine transcriptionally up-regulated expressions of acyl-coenzyme A synthetase (ACS) and carnitine palmitoyltransferase-I (CPT-I) by approximately 50% and 40%, respectively, compared with genistein alone. However, the up-regulation of CPT-I did not directly reflect the enzyme activity of CPT-I. On the other hand, the effects of genistein and genistein with carnitine on the expressions of ACS and CPT-I in muscle were not significant. Our study suggests that genistein with carnitine exerts anti-obesity effects, probably by modulating peroxisome proliferator-activated receptor-associated genes. However, further work is needed to elucidate the possible mechanisms by which genistein and carnitine intervene.
大豆制品主要由蛋白质、异黄酮等植物化学物质、大豆脂质和碳水化合物组成。目前尚不清楚是单一成分还是多种生物活性化合物的联合作用导致了大豆的有益特性。我们研究了膳食染料木黄酮（主要的大豆异黄酮）单独以及与左旋肉碱联合使用的效果，以评估对喂食高脂饮食（HD）的C57Bl/6J小鼠故意诱导的以胰岛素抵抗和肥胖为特征的糖尿病前期状态可能产生的协同作用。在仅喂食高脂饮食的组中，相对于总体重，腹部和背部脂肪显著高于包括喂食正常饮食（ND）的其他组。在高脂饮食组中，高脂饮食加染料木黄酮（HD+G）组和高脂饮食加染料木黄酮加左旋肉碱（HD+G+C）组的最终体重增加低于对照组（仅高脂饮食组）。特别是在肝脏中，结果表明，与单独使用染料木黄酮相比，染料木黄酮与肉碱联合使用可使酰基辅酶A合成酶（ACS）和肉碱棕榈酰转移酶-I（CPT-I）的转录表达分别上调约50%和40%。然而，CPT-I的上调并未直接反映CPT-I的酶活性。另一方面，染料木黄酮以及染料木黄酮与肉碱联合使用对肌肉中ACS和CPT-I表达的影响不显著。我们的研究表明，染料木黄酮与肉碱联合使用可能通过调节过氧化物酶体增殖物激活受体相关基因发挥抗肥胖作用。然而，需要进一步的研究来阐明染料木黄酮和肉碱干预的可能机制。
