Dillon N, Kollias G, Grosveld F, Williams J G
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, UK.
Nucleic Acids Res. 1991 Nov 25;19(22):6227-30. doi: 10.1093/nar/19.22.6227.
Transgenic mice were generated which carried the adult alpha and beta-globin genes and the major tadpole specific beta-globin gene of Xenopus laevis. The adult specific alpha and beta genes were found to express in erythroid tissues in adult mice, while the major tadpole specific beta gene (beta T1) was expressed in blood from 12.5 day embryos. The pattern of expression of the beta T1 gene during mouse development was consistent with its being regulated as an embryonic globin gene in the mouse. This observation suggests that some of the factors mediating globin switching have been conserved during the evolution of modern amphibia and mammals and raises interesting questions concerning the evolution of vertebrate globin gene switching.
生成了携带成年小鼠α和β珠蛋白基因以及非洲爪蟾主要蝌蚪特异性β珠蛋白基因的转基因小鼠。发现成年特异性α和β基因在成年小鼠的红系组织中表达，而主要蝌蚪特异性β基因（βT1）在12.5天胚胎的血液中表达。βT1基因在小鼠发育过程中的表达模式与其在小鼠中作为胚胎珠蛋白基因受到调控一致。这一观察结果表明，在现代两栖动物和哺乳动物的进化过程中，一些介导珠蛋白转换的因子得以保留，并引发了有关脊椎动物珠蛋白基因转换进化的有趣问题。
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