Meyerhof W, Klinger-Mitropoulos S, Stalder J, Weber R, Knöchel W
Nucleic Acids Res. 1984 Oct 25;12(20):7705-19. doi: 10.1093/nar/12.20.7705.
We present the complete nucleotide sequence of the larval beta I-globin gene of Xenopus laevis including 240 nucleotides of the 5' flanking region and 594 nucleotides beyond the polyadenylation site. The site of transcription initiation was mapped by S1 nuclease, and the site of polyadenylation was determined by comparison with corresponding cDNA clones. The larval Xenopus beta I-gene shows the same internal structure as the beta-globin genes of higher vertebrates, viz. 3 exons interrupted by 2 intervening sequences. The first intervening sequence, which is of exceptional length, spans over 564 nucleotides and interrupts the coding sequence at amino acid 30, whereas the second one comprises 968 nucleotides and is located between the amino acids 104 and 105. The second intervening sequence contains a long inverted repeat of almost perfect homology. The 5' flanking region contains a TATA- and a CAAT-box at positions -33 and -58, respectively. An additional TATA-box is located at -197 and two more CAAT-boxes occur at positions -105 and -237.
我们展示了非洲爪蟾幼虫βI-珠蛋白基因的完整核苷酸序列，包括5'侧翼区的240个核苷酸以及多聚腺苷酸化位点下游的594个核苷酸。转录起始位点通过S1核酸酶定位，多聚腺苷酸化位点通过与相应的cDNA克隆比较确定。非洲爪蟾幼虫βI-基因与高等脊椎动物的β-珠蛋白基因具有相同的内部结构，即3个外显子被2个间隔序列打断。第一个间隔序列长度异常，跨越564个核苷酸，在氨基酸30处打断编码序列，而第二个间隔序列包含968个核苷酸，位于氨基酸104和105之间。第二个间隔序列包含一个几乎完全同源的长反向重复序列。5'侧翼区在-33和-58位置分别含有一个TATA盒和一个CAAT盒。另一个TATA盒位于-197，另外两个CAAT盒出现在-105和-237位置。
