Hosbach H A, Wyler T, Weber R
Cell. 1983 Jan;32(1):45-53. doi: 10.1016/0092-8674(83)90495-6.
Clones containing nine different larval and adult globin genes have been isolated from two genomic libraries of Xenopus laevis. They encompass three distinct DNA regions: a 70 kb region containing five genes in the order 5'-alpha Lla-alpha Llb-alpha Al-beta Al-beta Lla-3', all with the same transcriptional polarity; a 40 kb region with three genes, 5'-alpha Llla-alpha Lllb-alpha All-3', again with the same polarity; and a 10 kb segment comprising the beta Llla gene only. The beta All gene has not been found in our libraries. Genetic analysis has revealed two more beta L genes (beta Lllb, beta Lllb). Globin-like sequences have also been isolated, but have not been further characterized. The X. laevis globin gene family thus consists of 12 genes arranged in two clusters, each containing larval and adult alpha and beta genes in a unique, "symmetrical" arrangement. Electron microscopic analysis has revealed that Xenopus globin genes also comprise three exons and two introns, but differ from the globin genes of higher vertebrates in that the introns are much larger.
已从非洲爪蟾的两个基因组文库中分离出包含九个不同幼虫和成年珠蛋白基因的克隆。它们涵盖三个不同的DNA区域：一个70 kb的区域，包含五个基因，顺序为5'-αLla-αLlb-αAl-βAl-βLla-3'，所有基因具有相同的转录极性；一个40 kb的区域，有三个基因，5'-αLlla-αLllb-αAll-3'，同样具有相同的极性；以及一个仅包含βLlla基因的10 kb片段。在我们的文库中未发现βAll基因。遗传分析还揭示了另外两个βL基因（βLllb、βLllb）。也分离出了类珠蛋白序列，但尚未进一步表征。因此，非洲爪蟾珠蛋白基因家族由12个基因组成，排列成两个簇，每个簇都以独特的“对称”排列方式包含幼虫和成年α和β基因。电子显微镜分析表明，非洲爪蟾珠蛋白基因也由三个外显子和两个内含子组成，但与高等脊椎动物的珠蛋白基因不同，其内含子要大得多。
