近临界 CO₂ 中的酶反应：压力对酪氨酸酶去除苯酚的影响。

Enzymatic reactions in near critical CO₂: The effect of pressure on phenol removal by tyrosinase.

机构信息

School of Chemistry, Universidade Federal do Rio de Janeiro, CT, Bl. E, Cidade Universitária, 21949-900, Rio de Janeiro, RJ, Brazil.

Chemical and Biological Engineering Department, Instituto Superior Técnico, Avenida Rovisco Pais, 1049-001 Lisboa, Portugal.

出版信息

Int J Mol Sci. 2009 Dec 1;10(12):5217-5223. doi: 10.3390/ijms10125217.

DOI:10.3390/ijms10125217

PMID:20054468

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2801991/

Abstract

The use of enzymes in supercritical CO(2) (SCCO(2)) has received extensive attention in recent years. Biocatalysts have the advantage of substrate specificity and SCCO(2) offers several advantages over liquid solvents. This work deals with the utilization of SCCO(2) as a medium for the enzymatic removal of phenol from aqueous solutions using tyrosinase. Since the presence of oxygen is crucial for the enzyme-catalyzed oxidation, the substantial solvating power of SCCO(2) makes it a promising medium for such reactions. The conversion of phenol was higher at 10 MPa. Under near critical conditions (7 MPa, 35 degrees C), the addition of air at 5 x 10(5) Pa of pressure improved phenol removal.

摘要

近年来，超临界二氧化碳（SCCO2）中酶的应用受到了广泛关注。生物催化剂具有底物特异性的优势，而 SCCO2 相对于液体溶剂具有多项优势。本工作利用超临界二氧化碳作为介质，使用酪氨酸酶从水溶液中酶促去除苯酚。由于氧气的存在对酶催化氧化至关重要，因此 SCCO2 的强溶剂化能力使其成为此类反应的有前途的介质。在 10 MPa 时，苯酚的转化率更高。在近临界条件（7 MPa，35°C）下，在 5 x 10(5) Pa 的压力下添加空气可以提高苯酚的去除率。