Bryanskaya Alla V, Malup Tatyana K, Lazareva Elena V, Taran Oxana P, Rozanov Alexey S, Efimov Vadim M, Peltek Sergey E
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 10 Prospekt Lavrentyeva, Novosibirsk, 630090, Russia.
V.S. Sobolev Institute of Geology and Mineralogy, Siberian Branch of Russian Academy of Sciences, 3 Prospekt Akademika Koptyuga, Novosibirsk, 630090, Russia.
BMC Microbiol. 2016 Jan 27;16 Suppl 1(Suppl 1):4. doi: 10.1186/s12866-015-0618-y.
Nothing is currently known about microbial composition of saline lakes of the Novosibirsk region and its dependence on physical-chemical parameters of waters. We studied the structure of microbial communities of saline lakes of the Novosibirsk region and the effect of physical-chemical parameters of waters on microbial communities of these lakes.
According to the ion content, the lakes were classified either as chloride or chloride-sulfate types. Water salinity ranges from 4.3 to 290 g L(-1). Many diverse microbial communities were found. Filamentous and colonial Cyanobacteria of the genera Scytonema, Aphanocapsa, and/or filamentous Algae dominated in littoral communities. Spatial and temporal organization of planktonic microbial communities and the quantities of Archaea and Bacteria were investigated using fluorescent in situ hybridization. We have found that the dominant planktonic component is represented by Archaea, or, less frequently, by Bacteria. Various phylogenetic groups (Bacteria, Archaea, Algae, and Cyanobacteria) are nonuniformly distributed. The principal component analysis was used to detect environmental factors that affect microorganism abundance. We found the principal components responsible for 71.1 % of the observed variation. It was demonstrated that two-block partial least squares was a better method than principal component analysis for analysis of the data. We observed general relationships between microbial abundance and water salinity.
We have performed the first-ever study of the structure of the microbial communities of eleven saline lakes in the Novosibirsk region along with their physical-chemical parameters of waters. Our study demonstrates that saline lakes in the Novosibirsk region contain a unique microbial communities that may become a prolific source of microorganisms for fundamental and applied studies in various fields of ecology, microbiology, geochemistry, and biotechnology, and deserve further metagenomic investigation.
目前对新西伯利亚地区盐湖的微生物组成及其对水体理化参数的依赖性尚不清楚。我们研究了新西伯利亚地区盐湖微生物群落的结构以及水体理化参数对这些湖泊微生物群落的影响。
根据离子含量，这些湖泊被分类为氯化物型或氯化物 - 硫酸盐型。水盐度范围为4.3至290 g L⁻¹。发现了许多不同的微生物群落。席藻属、隐球藻属的丝状和群体蓝细菌，和/或丝状藻类在沿岸群落中占主导地位。使用荧光原位杂交技术研究了浮游微生物群落的空间和时间组织以及古菌和细菌的数量。我们发现浮游生物的主要成分是古菌，或者较少情况下是细菌。各种系统发育类群（细菌、古菌、藻类和蓝细菌）分布不均匀。主成分分析用于检测影响微生物丰度的环境因素。我们发现主成分解释了观察到的变异的71.1%。结果表明，对于数据分析，两阶段偏最小二乘法比主成分分析是更好的方法。我们观察到微生物丰度与水盐度之间的一般关系。
我们首次对新西伯利亚地区11个盐湖的微生物群落结构及其水体理化参数进行了研究。我们的研究表明，新西伯利亚地区的盐湖含有独特微生物群落，这可能成为生态学、微生物学、地球化学和生物技术等各个领域基础和应用研究的丰富微生物来源，值得进一步进行宏基因组学研究。
